2.8 Wind tunnel bioassays
A wind tunnel modified from Hee and Tan (Hee and Tan, 1998) was used to assess the ability of moth to respond to olfactory stimuli. The wind tunnel was constructed of a transparent polyacetate sheet rolled into a cylindrical tube (150 × 30 cm diameter) with ends forming the big plastic box for placing moths (50 × 50 × 50 cm). An electric fan was used to generate a continuous laminar airflow at a speed of 20 cm/s. Apple and sex pheromones mix solution (E10-16:Ald and Z10-16:Ald base on the ratio 9:1 were dissolved in hexane to 100 ng/μL) (Yasuhiko et al., 1982) were alternately placed at the point (at the downwind end) in the wind tunnel for attracting the moths. When the dark period started 2 h, thirty male/female virgin moths were chosen and placed in the plastic box for testing in dark period. After 1 h, a moth in the tunnel reach or close to the apple or sex pheromones was counted, and each bouquet of female moth or male moth test repeat 5 times.
2.9 Interaction predicted analysis and gene selection
Interaction analysis of GOBP1 and GOBP2 was based on the STRING database (https://string-db.org/), including known and predicted protein-protein interactions. GOBP2 ofBombyx mori as a reference template to construct a network interaction map. Based on the results of the interaction, we selected the proteins that might interact with GOBPs and constructed a phylogenetic tree based on the predicted interactions between Bombyx mori protein and the identified genes of the yellow peach moth.