2.5 Correlation analysis
Correlation analysis was carried out between
transcriptomic analysis revealed
differentially expressed genes (DEGs) and analysis of differentially
expressed proteins (DEPs). Functional annotation of
transcripts
and proteins data were searched using BLASTX against the non-redundant
(nr) NCBI protein database (Liu et al., 2012). The calculation of
unigene expression uses the FPKM method (Fragments Per kb per Million
reads); In addition, using Blast2GO (http://www.blast2go.org) (Conesa et
al., 2005), we predicted and classified functions of unigenes by
Clusters of EuKaryotic of orthologous groups (KOG) database (Ashburner
et al., 2000). In addition, the online Kyoto Encyclopedia of Genes and
Genomes (KEGG) Automatic Annotation Server (KAAS) was employed for KEGG
pathway enrichment analysis following the procedure of pathway
annotations for transcripts and proteins data (Kanehisa et al., 2008;
Shevchenko et al., 2008).