2.6 Gene sequences verify and qPCR detection
Total RNA was reverse transcribed to cDNA using RTTMAll-in-One Master Mix Kit (Herogen Biotech, Shanghai, China) according
to the protocol of the manufacturer, then PCR technology was used to
amplify the selected gene sequences in two species. qPCR (quantitative
real-time PCR) experiments were conducted with actin as an internal
ribosomal protein RP49 (Yang et al., 2017), and calculations were
performed as described previously (Jing et al., 2020). All primer
sequences are given in
(Supplementary
Table 1).