3.2 Correlation analysis between DEGs and DEPs
Totally, 74611 DEGs and 391 DEPs were correlated and analyzed according to their difference multiples (Figure 1A). GO analysis was used to classify the Biological process, Molecular function and Cellular components after the transcriptomic and proteomic correlate analysis (Supplementary Figure 1A). The results showed that these genes were mainly closely related to metabolism and redox (Supplementary Figure 1B and C). From KEGG annotation, the data as well as mainly closely related to metabolism and redox (Supplementary Figure 2). After correlation analysis, we found that 249 of the transcriptome and proteome data are overlapped, and 142 proteins are differential proteins after correlation analysis (Figure 1C). Next, we selected all the proteins (30 proteins) related to digestion and metabolism from 142 different proteins for further study (Supplementary Table 3). Then based on whether using PCR can obtain the open reading frame (ORF) of the genes form the transcriptome data, a total of 9 proteins were selected (Table 3).