2.5 Correlation analysis
Correlation analysis was carried out between transcriptomic analysis revealed differentially expressed genes (DEGs) and analysis of differentially expressed proteins (DEPs). Functional annotation of transcripts and proteins data were searched using BLASTX against the non-redundant (nr) NCBI protein database (Liu et al., 2012). The calculation of unigene expression uses the FPKM method (Fragments Per kb per Million reads); In addition, using Blast2GO (http://www.blast2go.org) (Conesa et al., 2005), we predicted and classified functions of unigenes by Clusters of EuKaryotic of orthologous groups (KOG) database (Ashburner et al., 2000). In addition, the online Kyoto Encyclopedia of Genes and Genomes (KEGG) Automatic Annotation Server (KAAS) was employed for KEGG pathway enrichment analysis following the procedure of pathway annotations for transcripts and proteins data (Kanehisa et al., 2008; Shevchenko et al., 2008).