Abstract
Although Conogethes
punctiferalis and Conogethes pinicolalis have reported on
morphology and molecular identification, the molecular biology
differences between the two species in different diets are still
unknown. In this work, we investigated the difference in the biological
process of the two species by the methods of transcriptomics,
proteomics, metabolomics, and bioinformatics. The results showed that
the expression of 74,611 mRNA in the transcriptome, 391 proteins in the
proteome, and 218 metabolites in the metabolome had been significantly
changed between the two species, and the KEGG results showed the data
were mainly closely related to metabolism and redox.
Moreover, based on integrating
system-omics data, we found that the α-amylase and CYP6AE76 gene have
mutations between the two species. Different sequence analysis results
may differ significantly in their exact substrate preference and product
profile, which indirectly leads to the different detoxification or
metabolism ability, and the gene expression of relevant was also
confirmed by the qPCR and enzyme activity test. These findings in the
two species and integrated networks provide beneficial information for
further exploring the molecular mechanism of polyphagous and
oligophagous.