3.4 Detection of the expression levels of two genes
The results show that both genes
(α-amylase and CYP6AE76) are
highly expressed in C. punctiferalis and reached a significant
level compared with C. pinicolalis (Figure 3). This result is the
same as the trend in the transcriptome data (Figure 1C).
3.5
α-amylase andCYP6AE76
activity
Four proteins were successfully expressed in E. coli(Supplementary Figure 3), and the
protein obtained after subsequent purification was used for the enzyme
activity test. The amount of substrate, ethylidene-pNP-G7, cleaved by
the purified α-amylase from C. pinicolalis showed higher activity
than C. punctiferalis (Figure 4A). Furthmore, conversion of p-NA
to p-NP by CYP6AE76 was
significantly higher in C. punctiferalis (Figure 4B).