2.6 Gene sequences verify and qPCR detection
Total RNA was reverse transcribed to cDNA using RTTMAll-in-One Master Mix Kit (Herogen Biotech, Shanghai, China) according to the protocol of the manufacturer, then PCR technology was used to amplify the selected gene sequences in two species. qPCR (quantitative real-time PCR) experiments were conducted with actin as an internal ribosomal protein RP49 (Yang et al., 2017), and calculations were performed as described previously (Jing et al., 2020). All primer sequences are given in (Supplementary Table 1).