Figure legends
Figure 1. Interaction analysis of DEGs and DEPs. (A)Correction plot analysis based on DEGs and DEPs. (B) Venn
diagram of DEGs and DEPs. (C) Heat map
based on FPKM value of DEGs and
DEPs.
Figure 2 . Diagram of the secondary and tertiary structure of
the mutant gene. (A) Comparison of amino acid sequences of
alpha-amylases and CYP6AE76.
Homologous sequence regions 1, 2,
3 and 4 are surrounded by purple rectangles. The amino acid sequence
described above the rectangular regions was taken as representative of
regions 1 to 4, respectively.
Active sites and those of
substrate binding proposed by Matsuura et al. (Matsumura et al., 1984)
for Taka-amylase A from are indicated by the purple triangle and black
oval, respectively; SRS is represented in green line boxes, heme-binding
signature motif (FxxGxxxCxG) (black-dotted rectangular frame), helix C
motif (WxxxR) (navy blue box), and PxPF motif are in lightboxes.(B) superimpositions of predicted models of α-amylase and
CYP6AE76 from C. pinicolalis and C. punctiferalis with
their respective templates.
Figure 3. Gene expression
of α-amylase and P450 monooxygenase CYP6AE76 in C. pinicolalisand C. punctiferalis . (A) The relative expression of
α-amylase in C. pinicolalis and C. punctiferalis .(B) The relative expression of P450 monooxygenase CYP6AE76 inC. pinicolalis and C. punctiferalis . The gene expression
level between the two species was statistically significant
(t -test, ***P < 0.001).
Figure 4. The comparison of enzymatic activity of recombinant
α-amylase and CYP6AE76 from two species. (A) The amount of
ethylidene-pNP-G7 (substrate) cleaved by the purified
α-amylase from C.
pinicolalis and C.
punctiferalis . (B) Conversion of p-nitroanisole to
p-nitrophenol in by recombinant cytochrome P450 from C.
pinicolalis and C. punctiferalis . The α-amylase and CYP6AE76
enzyme activities were statistically significant (t -test,**P <0.01).
Figure 5. Association analysis the different genes between the
two species (C. pinicolalis vs C. punctiferalis ).(A) Volcano map of all differential genes;(B) Top 20 down- and
up-accumulated metabolites between the two species.
Figure S1. Transcriptome and proteomics association analysis.(A) Differential gene GO enrichment. (B) and(C) Differential gene GO enrichment of biological process and
molecular function, respectively.
Figure S2. KEGG pathway enrichment scatter plot of the
different genes.
Figure S3. Candidate gene recombinant expression protein.(A) SDS-PAGE analysis of purified recombinant Candidate
proteins. The four SDS-PAGE diagrams are Coomassie Blue staining of
purified α-amylase (1 and 2) and CYP6AE76 (3 and 4) protein samples from
left to right in C. pinicolalis vs C. punctiferalis ,
respectively. M: Protein marker; Lane 1,2,5,6,9,10,13 and 14: Sonicated
bacterial pellet; 3,4,7,8,11,12,15 and 16: purified protein.(B) Western blot analysis of α-amylase (Line 1 and 2) and
CYP6AE76 (Line 3 and 4) protein samples from left to right in C.
pinicolalis vs C. punctiferalis , respectively. M: Protein
marker.
Figure S4. Metabolite KEGG classification map.
Figure S5. KEGG pathway enrichment scatter plot of the
different metabolite.