3.4 Detection of the expression levels of two genes
The results show that both genes (α-amylase and CYP6AE76) are highly expressed in C. punctiferalis and reached a significant level compared with C. pinicolalis (Figure 3). This result is the same as the trend in the transcriptome data (Figure 1C).
3.5 α-amylase andCYP6AE76 activity
Four proteins were successfully expressed in E. coli(Supplementary Figure 3), and the protein obtained after subsequent purification was used for the enzyme activity test. The amount of substrate, ethylidene-pNP-G7, cleaved by the purified α-amylase from C. pinicolalis showed higher activity than C. punctiferalis (Figure 4A). Furthmore, conversion of p-NA to p-NP by CYP6AE76 was significantly higher in C. punctiferalis (Figure 4B).