5. Conclusion
To summarize, the present study showed that the mRNA levels, proteins
and metabolites had significantly altered
in polyphagous C.
punctiferalis and oligophagous C. pinicolalis by the multi-omics
techniques and all the data were mainly closely related to metabolism
and redox. In particular, the mutations of α-amylase and CYP6AE76 gene
lead to differences in gene expression levels and enzyme activities,
resulting from long-term evolutionary selection between the two species.
These findings increase our understanding of the two species and offer
new perspectives for revealing the molecular mechanisms of polyphagous
and oligophagous.