Figure 1
Ixazomib inhibits growth and colony formation of esophageal squamous carcinoma cells. (A), Effect of ixazomib on the viability of breast cancer cells Kyse150, Kyse510, Kyse520, Eca109 and HECC. Cells were treated with vehicle or ixazomib (0.1, 1, 10 μM) for 24 h and 48h, the viability was detected with the CCK-8 kit. (B), ESCC cells were treated with various concentrations of ixazomib for 24 hours. Cell proliferation was determined by the CCK-8 assay. IC50 value of ixazomib in ESCC cell lines was then determined. (C, D), Efficacy of ixazomib on colony formation of ESCC cells. ESCC cells were treated with ixazomib (0.25, 0.5 μM) for 7days, and then fixed, stained and counted. Values are the means ± SEM (n = 3). *P < 0.05 or **P < 0.01 indicates significant differences from the vehicle group as assessed by a two-tailed unpaired Student’s t test.