Gene silencing using small interfering RNA (siRNA)
Kyse150 and Kyse510 cells were transfected using Lipofectamine 2000 (Invitrogen, life technologies) with siRNA mixture targeting NOXA or c-Myc for 48 h and further treated with ixazomib (1 μM) for another 48 h. Then cells were collected for cell viability assay. The sequences of the siRNA are as follows:
siNOXA#1: GUAAUUAUUGACACAUUUC
siNOXA#2: GGUGCACGUUUCAUCAAUUUG
sic-Myc#1: AACGUUAGCUUCACCAACA
sic-Myc#2: CGAGCUAAAACGGAGCUUU