Cell culture
The human esophageal squamous carcinoma cell lines KYSE 150, KYSE 510, KYSE520, ECa109, TE1 and human esophageal epithelial cells lines HECC were purchased from American Type Culture Collection. KYSE 150, KYSE520, ECa109 and TE1 were cultured in Roswell Park Memorial Institute-1640 medium (RPMI-1640, Hyclone). KYSE 510 were cultured in 50% F12 medium (Hyclone) and 50% RPMI-1640 mixed medium. HECC cultured in high glucose Minimum Essential Medium (MEM, Hyclone). Both media were supplemented with 10% fetal bovine serum (FBS, Yeasen Biological Teachnology Co., Ltd), and 100 U/ml penicillin/streptomycin (Wisent) at 37 °C in a humidified incubator with 5% CO2 and 95% air.