Esophageal squamous carcinoma xenografts
Human esophageal squamous carcinoma cell xenografts were generated by
subcutaneously injecting 2×106 KYSE150 cells suspended
with PBS/Matrigel into the right flank region of 6-week-old BALB/c nude
mice (Zhang et al. , 2016). Tumor
growth was monitored once per 5-days after implantation. When
subcutaneous tumors reached an average size of>100 mm3,
mice were treatments intragastrically with ixazomib or vehicle (5%
DMSO+45% PEG300+ddH2O) were then initiated. During the study, Mice were
weighed per 10-days during and after the drug-treatments. Tumor volumes
were calculated using the formula V=
length×width2×0.5, where length and width represent
the longer and shorter tumor diameters, respectively. All procedures
were performed in accordance to approved protocols by the Fudan
University Institutional Animal Care and Use Committee. Every effort was
made to minimize potential distress, pain, or discomfort to the animals
throughout all experiments.