Figure 4
NOXA participates in ixazomib induced proliferation inhibition in ESCC cells. (A) Kyse150 cell treated with or without 1μM ixazomib for 48h. The expression of ER stress related gene was examined by RT-PCR. (B, C) After transfected with the NOXA siRNA for 48 h, Kyse150 and Kyse510 cells were further treated with ixazomib (1μmol) for 48 h and the cell viability and colony formation were examined. Values are the means ± SEM (n = 5-6). *P < 0.05 or **P < 0.01 indicates significant differences from the vehicle group as assessed by a two-tailed unpaired Student’s t test.