Western blot analysis
Western blotting was done as described previously (Zhang et al. , 2019). Bcl-2, NOXA, c-Myc (Cell Signaling Technology), were used as primary antibodies. These primary antibodies were followed by appropriate secondary antibodies conjugated to horseradish peroxidase. Antibody-protein complexes were detected using enhanced chemiluminescence (ECL) immunoblotting detection reagent. The signals were analyzed using a LAS-3000 image analyzer and MultiGauge software (Fuji Film).