Western blot analysis
Western blotting was done as described previously
(Zhang et al. , 2019). Bcl-2, NOXA,
c-Myc (Cell Signaling Technology), were used as primary antibodies.
These primary antibodies were followed by appropriate secondary
antibodies conjugated to horseradish peroxidase. Antibody-protein
complexes were detected using enhanced chemiluminescence (ECL)
immunoblotting detection reagent. The signals were analyzed using a
LAS-3000 image analyzer and MultiGauge software (Fuji Film).