Genetic and phylogenetic analysis
The 91 complete S gene sequences of PEDV obtained in this study have
been uploaded to GenBank with accession numbers from MZ160999 to
MZ161089. Twenty-four reference sequences retrieved from the NCBI
nucleotide database and the 91 identified sequences were used for a
phylogenetic analysis. Multiple nucleotide sequence alignments were
performed with Clustal W algorithm using MEGA
6.0 software. A phylogenetic tree
based on S gene sequence was constructed by the neighbor-joining method
using MEGA 6.0 software, with bootstrap values calculated for each node
from 1,000 replicates (Hu et al., 2015;
Kumar, Stecher, Li, Knyaz, & Tamura,
2018). The evolutionary tree was further visualized by iTOL v4 software
(Letunic & Bork, 2019).