Abbreviations:
SI, Sucrose isomerase; DSF, Differential Scanning Fluorimetry; WT, Wild type enzyme; MD, molecular dynamic simulation; C.glutamium 13032,Corynebacterinum glutamicum ATCC13032 ; RMSD, Root mean square deviation; RMSF, root mean square fluctuation; IPTG, isopropyl-β-D-thiogalactopyranoside; ΔΔG, the relative folding free energy changes;  SDS−PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; HPLC, high-performance liquid chromatography; RID, refractive index detector;Tm , melting temperature; VDW, Vander Waals forces.