2.8 Determination of Kinetic Parameters
Kinetic Parameters of these purified enzymes were determined under standard assay conditions with sucrose as a substrate. The sucrose substrate concentrations were as follows: 14.6, 29.2, 58.4, 102, 146, 234, 292 and 584 mM, respectively. Then, Vmax and Km values were determined through regression fitting of experimental data using GraphPad Prism 8.0.