Abbreviations:
SI, Sucrose isomerase; DSF, Differential Scanning Fluorimetry; WT, Wild
type enzyme; MD, molecular dynamic simulation; C.glutamium 13032,Corynebacterinum glutamicum ATCC13032 ; RMSD, Root mean square
deviation; RMSF, root mean square fluctuation; IPTG,
isopropyl-β-D-thiogalactopyranoside; ΔΔG, the relative folding free
energy changes; SDS−PAGE, sodium dodecyl sulfate polyacrylamide gel
electrophoresis; HPLC,
high-performance liquid
chromatography; RID, refractive index detector;Tm , melting temperature; VDW, Vander Waals
forces.