2.8 Determination of Kinetic Parameters
Kinetic Parameters of these purified enzymes were determined under
standard assay conditions with sucrose as a substrate. The sucrose
substrate concentrations were as follows: 14.6, 29.2, 58.4, 102, 146,
234, 292 and 584 mM, respectively. Then, Vmax and
Km values were determined through regression fitting of
experimental data using GraphPad Prism 8.0.