Figure legends
Figure 1. (A) Distribution of the mutations in protein PDSI.
The residues at the mutational sites are shown as spheres. (B) SDS-PAGE
shows the expression of mutants. Lane M, protein marker; Lane1, C.
glutamicum /pXMJ19/pdsiWT; Lane2-12, C. glutamicum /pXMJ19/pdsi
(E76R, A100E, G152P, I205M, V280L, S328F, S499F, S563R, S563L, N578M,
V280L/S499F). (C) The residual activity of wild-type (WT) and its
mutants after incubation at 45°C for 20 min.
Figure 2. Effects of pH and temperature on the activity of WT
and its mutants. (A) The optimal reaction pH of WT and its mutants. (B)
The optimal reaction temperature of WT and its mutants. (C) The
thermostability of WT and the mutants at 45°C. (D) TheTm of WT and its mutants measured by DSF.