2.9 Differential scanning fluorescence (DSF) assay
The DSF procedure used in this research was slightly modified according to previous report.[20] TheTm values were determined through monitoring the maximum relative fluorescence intensity after incubating the purified protein and SYPRO Orange dye together in PCR microplate at increasing temperatures in the real-time PCR machine. Tmvalues were measured in the rage of 25~ 95°C at a rate of 1 °C/min. Three parallel samples were determined