2.1 Reagents and enzymes
Sucrose, isomaltulose, HPLC-grade acetonitrile were bought from Aladdin
(Shanghai, China). PrimSTAR Max DNA Polymerase, restriction enzymes,
protein markers and Dpn I were obtained from TaKaRa (Dalian,
China). Plasmid Mini Preparation Kit and BCA Protein Assay Kit were
provided by Beyotime (Shanghai, China).
Isopropyl-β-D-thiogalactopyranoside (IPTG) and chloramphenicol was
supplied by Yuanye Bio-Technology (Shanghai, China). All other reagents
were purchased from Sinopharm Chemical Reagent (Shanghai, China) unless
otherwise noted.