2.11 HPLC analysis
Samples were determined by HPLC (Agilent 1260, USA) system equipped with
a refractive index detector (RID) and separated by a NH2column (250 mm × 4.6 mm). The mobile phase was 80 % acetonitrile with
the flow rate of 1.0 mL•min-1 at 30°C, and RID
temperature was controlled at 35°C. The amounts of sugar concentration
were calculated via peak areas.
Results and Discussion