2.5 Subcellular Localization
The full-length coding sequences of CsHSP24.6 and CspTAC5lacking its termination codon were separately cloned into the entry vector pDONR207 using the Gateway BP Enzyme mix (Invitrogen) with specific primers. The plasmids newly extracted after sequencing were then introduced into the destination vector named pGWB5 [fused with green fluorescent protein (GFP) at the C-terminal] using the LR Clonase enzyme (Invitrogen). After sequencing, the plasmids pGWB5-CsHSP24.6 and pGWB5-CspTAC5 were separately transformed into the Agrobacterium tumefaciens strain GV3101 as described above. Selection of a positive colony for cultivation and infiltration of Nicotiana benthamiana was performed following a reported protocol42. After infection for 48 h, the leaves were examined by using an Olympus FV1000confocal microscope (Japan).