2.9 Leaf Area Measurement
Please see the reference method at the following website:
http://www.medtranslation.cn/yixuetuku/20171013/130543.html.
First, a leaf was taken from the leaf base by cutting with a scissors.
The length and width of each leaf were measured with a ruler and
recorded. A rectangular piece of paper that was larger than the leaf was
chosen. The length L , width W , and mass M of the
paper were recorded. The paper area was calculated as A =L × W . The leaf was fixed on the paper, and the shape of
the leaf was traced using a pencil. The paper was then cut into the
shape of the leaf with scissors. The leaf was removed, and the mass of
the cut leaf-shaped paper was measured on the electronic balance and
recorded as m . The leaf area a was then calculated asa = A × (m /M ) and the shape coefficient wask = a /(L × W ). The shape coefficient of 10
leaves was calculated, and an average value was determined. For
subsequent measurements, the length and width of the leaves were
measured with a ruler, and the product of these were multiplied by the
shape factor to obtain the leaf area. The data and shape factors we
measured are shown in the Table S6 .