2.3 Phylogenetic Tree Construction and Conserved Motif Analysis
The amino acid sequences of the sHSP s of A. thaliana and
other plants were downloaded from the TAIR
(http://www.arabidopsis.org/)
and NCBI databases; unrooted neighbor-joining phylogenetic trees were
constructed using MEGA 7.0 and EvolView
(http://www.evolgenius.info/evolview).
The MEME program (version 4.10.0, http://meme-suite.org/) was used to
identify the conserved protein motifs of all CssHSP s with the
following parameters: number of repetitions was any, maximum number of
motifs was 10, and optimum motif widths were from 6 to 200 amino acid
residues.