3.5 CsHSP24.6 and CspTAC5 Jointly Resist High
Temperature and Light Intensity
Transgenic and WT plants were used to determine whether overexpression
of CsHSP24.6 or CspTAC5 improved tolerance to adverse
temperatures and light intensities.
Six-week-old 35S::CsHSP24.6and 35S::CspTAC5 transgenic plants and WT control plants were
analyzed using a plant chlorophyll fluorometer after high-temperature
and light treatments.
The fluorescence parameter Fv/Fm represents the maximum quantum yield of
PSII, which is related to the potential maximum photosynthetic capacity
of the plant. Results for Chl fluorescence imaging and Fv/Fm of the
transgenic lines were similar to those of WT under normal growth
conditions (Figure 6 ). However, upon exposure to
high-temperature and high-light stresses, WT exhibited impairment in Chl
fluorescence images and significantly lower Fv/Fm compared with the
transgenic lines. These results indicate that overexpression ofCsHSP24.6 or CspTAC5 enhanced the heat and light tolerance
of the transgenic plants.