2.7 Bimolecular Fluorescence Complementation
Bimolecular fluorescence complementation (BiFC) assay was performed according to a previous study44. Full-lengthCsHSP24.6 and CspTAC5 cDNAs were subcloned into pCambia-CYFP and pCambia-NYFP, respectively, and plasmids were co-transformed into protoplasts (for primers used for fusion constructs; see Table S6 online). Yellow fluorescent protein (YFP) fluorescence was imaged using a confocal laser scanning microscope (Leica SP8).