3.5 CsHSP24.6 and CspTAC5 Jointly Resist High Temperature and Light Intensity
Transgenic and WT plants were used to determine whether overexpression of CsHSP24.6 or CspTAC5 improved tolerance to adverse temperatures and light intensities. Six-week-old 35S::CsHSP24.6and 35S::CspTAC5 transgenic plants and WT control plants were analyzed using a plant chlorophyll fluorometer after high-temperature and light treatments.
The fluorescence parameter Fv/Fm represents the maximum quantum yield of PSII, which is related to the potential maximum photosynthetic capacity of the plant. Results for Chl fluorescence imaging and Fv/Fm of the transgenic lines were similar to those of WT under normal growth conditions (Figure 6 ). However, upon exposure to high-temperature and high-light stresses, WT exhibited impairment in Chl fluorescence images and significantly lower Fv/Fm compared with the transgenic lines. These results indicate that overexpression ofCsHSP24.6 or CspTAC5 enhanced the heat and light tolerance of the transgenic plants.