2.7 Bimolecular Fluorescence Complementation
Bimolecular fluorescence complementation (BiFC) assay was performed
according to a previous study44. Full-lengthCsHSP24.6 and CspTAC5 cDNAs were subcloned into
pCambia-CYFP and pCambia-NYFP, respectively, and plasmids were
co-transformed into protoplasts (for primers used for fusion constructs;
see Table S6 online). Yellow fluorescent protein (YFP) fluorescence was
imaged using
a
confocal laser scanning microscope (Leica SP8).