2.16. H&E staining and IHC
Tumours and tissue specimens were fixed and embedded in paraffin.
Sections were cut from the paraffin blocks. For IHC staining, samples
were carried out using the VECTASTAIN ABC kit (Vector). AntiāKi67
(1:250; Catalogue #ab15580, Abcam) was used as the primary antibody.
For H&E staining, samples were stained with haematoxylin and eosin
according to standard protocols.