2.14.
ELISA
Immunoglobulin levels in serum were measured by ELISA. The titres of
isotype-specific antibodies were determined for the binding of
NP-specific antibodies to NP5-BSA and
NP23-BSA on coated plates
according to the manufacturer’s
protocol (Southern Biotech). OD values at 450 nm were measured by a
plate reader (BioTek).