2.6. Immunofluorescence assay
SUDHL4 cells were exposed to WK500B for 24 h, fixed with 4%
paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with
1% BSA. The cells were incubated with the antibodies against BCL6 (CST,
14895) and SMRT (Invitrogen, MA1-842) overnight. Cells were then stained
with secondary antibodies and DAPI was used to visualize the nuclei.
Images were acquired on a confocal microscope (Leica).