2.2. BCL6 Protein and SMRT Peptide
The DNA segment coding for the BCL6 BTB domain (5-129) with mutations in
C8Q, C67R and C84N was cloned into a vector containing a C-terminal GST
tag. The construct was expressed in E. coli BL21 (DE3), and the culture
was incubated at 18 ℃ with 0.5 mM IPTG for 18 h.
BCL6BTB protein was purified by glutathione agarose
resin and dialyzed with PBS. The peptide SMRT (1414-1430) with a 6His
tag was synthesized by Abace Biology Company.