2.1. Luciferase reporter assay
The reporter construct (GAL4)5-TK-LUC and the
GAL4-DBD-BCL6BTB expression plasmid were kindly
provided by Dr. Ari Melnick (Weill Cornell Medical College, Department
of Haematology/Oncology, New York, NY, USA.) A TK- Renilla
luciferase plasmid was included as an internal control (Promega, WA,
USA). 293T cells were cotransfected with (GAL4)5-TK-LUC
and GAL4-DBD-BCL6BTB or GAL4-DBD plus a
TK- Renilla reporter construct using Lipofectamine 2000 (Thermo
Fisher Scientific). After transfection for 6 h, the cells were treated
with the compounds for 24 h. A dual luciferase assay was performed
according to the manufacturer’s guidelines (Promega, WA, USA).