Study design and participants
This study presents a retrospective evaluation of the 238 patients
admitted to Health Sciences University Samsun Training and Research
Hospital who applied with suspicion of COVID-19 between March 11 and May
30, 2020. PCR results are determined in two categories, PCR positive
group, and PCR negative group in line with The World Health
Organization’s guide titled ”Laboratory Testing for 2019 Novel
Coronavirus (2019-nCoV) in Suspected Human Cases” published on March 2,
2020 and TC Ministry of Health COVID-19 Guidelines.
All samples were studied with the SARS CoV-2 Double Gene RT-q PCR Kit
(BioSpeedy, Turkey) in accordance with the manufacturer’s instructions.
Briefly, after nucleic acid isolation in nasopharyngeal lavage/aspirate,
broncho alveolar lavage, nasopharyngeal suture, oropharyngeal swab and
sputum samples, detection by single-step reverse transcription (RT) and
Real-Time PCR targeting the ORF1ab and N gene regions were performed.
Extract ion and inhibition control were checked by targeting the human
RNase P gene as an internal control. Nucleic acid extraction was
validated with the vNAT buffer and this process was conducted without
any additional work during sample transfer. Nasopharyngeal swap
ororopharyngeal swab samples taken with swabs (dacronor polyester flock)
were placed in a sterile transport solution containing vNAT solution and
transferred. Reaction components 2X Prime Script mix 10µL, CVD Di Oligo
mix 5µL, template nucleic acid 5µL total reaction amount of 20µLvolume
was created. Qiagen Rotor Gene (Germany) Real-Time PCR instrument was
run on sigmoidal curves under 38 cycles and were evaluated as positive.
Prothrombin time (PT), activated partial thromboplastin time (aPTT),
D-dimer, International Normalized Ratio (INR) and platelet (PLT) levels
of 119 patients followed in COVID-19 services were taken from hospital
information management system. In addition, PT, aPTT, D-dimer, INR and
PLT levels of 119 individuals with COVID-19 PCR negative were taken.
D-dimer test was worked on a Beckman Coulter Au 680 device, PT, aPTT and
INR tests were worked at Siemens Ca-7000 device and platelet level was
worked in Beckman Coulter Dx-800 device by using suitable tubes and
kits.