ELISA (enzyme linked immunosorbent assay)
Concentration of IL-1β, IL-6, TNF-α and IL-10 in CFs culture supernatants were determined using corresponding ELISA a kit in accordance with the manufacturer’s instructions. Absorbance was measured at 450 nm using a microplate reader. Results were compared with a standard curve constructed by titrating standards respectively. The level of IL-1β, IL-6, TNF-α and IL-10 in cell culture supernatants, expressed as pg/ml, were standardized based on each corresponding standard curve.Samples tested in triplicate and each experiment was repeated 3 times inindependently.