ELISA (enzyme linked immunosorbent assay)
Concentration of IL-1β, IL-6, TNF-α and IL-10 in CFs culture
supernatants were determined using corresponding ELISA a kit in
accordance with the manufacturer’s instructions. Absorbance was measured
at 450 nm using a microplate reader. Results were compared with a
standard curve constructed by titrating standards respectively. The
level of IL-1β, IL-6, TNF-α and IL-10 in cell culture supernatants,
expressed as pg/ml, were standardized based on each corresponding
standard curve.Samples tested in triplicate and each experiment was
repeated 3 times inindependently.