Fig. 4. Changes of α-SMA expression after treatment with serelaxin. (A) Immunostaining of α-SMA. Bar = 100 µm. (A) Expression of α-SMA mRNA assessed with RT-PCR. (B) Expression of α-SMA assessed with Western blot. (C) The statistical result of α-SMA expression. Serelaxin inhibits α-SMA expression in TGF-β1-induced cells. *p < 0.05 versus control group,#p < 0.05 versus LPS group. †p < 0.05 versus LPS+serelaxin group.&p < 0.05 versus LPS+serelaxin+GW group.