Chemicals and Reagents
Serelaxin was purchased from Peprotech (Rocky Hill, NJ, USA). Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were purchased from GIBCO (MD, USA). Lipopolysaccharides (LPSs, Escherichia coli 055:B5) was obtained from Sigma-Aldrich (USA). Rat IL-1β, TNF-α, and IL-6 ELISA kits were obtained from R&D (USA), and rat IL-10 ELISA kit was purchased from Invitrogen (USA). Rabbit polyclonal antibodies for vimentin, α-SMA, MMP-2, MMP-9, Collagen Ⅰ and Collagen Ⅲ were purchased from Abcam (Cambridge, MA). Rabbit polyclonal antibodies for NF-κB p65 and phospho-NF-κB p65 (S276) were purchased from Cell Signaling Technology (Danvers, MA, USA). Rabbit polyclonal antibodies for phospho-IκB-α, and phosphorylated-IκB-α,PPAR-γ were purchased from Santa Cruz Biotechnology (CA, USA). GW9662 was from Cayman Chemical (Ann Arbor, MI, USA); Rabbit polyclonal antibody for β-actin was from Trevigen (USA). Alexa Fluor 594 conjuncted goat anti-mouse IgG and Alexa Fluor 488 conjuncted goat anti-rabbit were obtained from Jackson ImmunoResearch Laboratories (West Grove, PA) 4′,6-diamidino-2-phenylindole (DAPI) was purchased from Sigma-Aldrich (Saint Louis, MO). RNAiso Plus, PrimeScript RT MasterMix Perfect Real Time, and SYBR Premix Ex Taq II (Perfect Real Time) were purchased from Takara (Sangon Biotech, Shanghai).