Figure legends
Figure 1. Experimental design. Briefly, mice were subjected to
surgery and recovered for 7 days. Thereafter, FeCl3 was
stereotaxically injected into the somatosensory cortex, followed by
Fer-1 or vehicle. After the mice from different groups were waken up,
seizure scoring was carried out for 90 min according to Racing scale.
Then Electroencephalogram (EEG) recording from day 1 to day 3 after
FeCl3 injection was recorded, which was considered as
the acute stage of PTE. In the chronic stage of PTE (from day 19 to day
21 after FeCl3 injection), EEG recording was also
captured and meanwhile, the cognitive performance was evaluated via
novel object recognition (NOR) test (from day 24 to day 26 after
FeCl3 injection) and Morris Water Maze (MWM) test (from
day 29 to day 34 after FeCl3 injection). Additionally,
on the day 3 and day 34 after FeCl3 injection, brain
tissue samples were both harvested for biochemical detection or
pathological analysis. isc, injection in the somatosensory cortex.
Figure 2. Fer-1 has an anticonvulsant effect against
FeCl3-induced PTE. (A) Illustration of the injected
site for Fer-1 and the location of electrode implantation for EEG
recording; (B) Mean seizure scores after FeCl3 injection
at the interval time of 90 min; (C) Representative EEG recordings and
power spectrum density in the acute stage of PTE; (D) Quantification of
EEG power spectra from day 1 to day 3 after FeCl3injection in different groups. The baseline EEG trace was monitored 30
min before FeCl3 injection; (E-F) Cumulative analysis of
baseline power and total power; (G) Effects of Fer-1 on the epileptiform
spike frequency (marked with spikes per 10 min) from day 1 to day 3
after FeCl3 injection in different groups; (H)
Indication of heat map of seizure activity over time (per day) per mouse
treated with either vehicle or Fer-1 in the acute stage of PTE; (I-K)
Effects of Fer-1 on the seizure onset, number of seizures and time in
seizure in FeCl3-induced PTE. Data were shown as mean ±
SEM (n=6), *p<0.05 versus control group, **p<0.01
versus control group, ***p<0.001 versus control,#p<0.05 versus
FeCl3+Vehicle group,##p<0.01 versus
FeCl3+Vehicle group and###p<0.001 versus
FeCl3+Vehicle group.