Figure legends
Figure 1. Experimental design. Briefly, mice were subjected to surgery and recovered for 7 days. Thereafter, FeCl3 was stereotaxically injected into the somatosensory cortex, followed by Fer-1 or vehicle. After the mice from different groups were waken up, seizure scoring was carried out for 90 min according to Racing scale. Then Electroencephalogram (EEG) recording from day 1 to day 3 after FeCl3 injection was recorded, which was considered as the acute stage of PTE. In the chronic stage of PTE (from day 19 to day 21 after FeCl3 injection), EEG recording was also captured and meanwhile, the cognitive performance was evaluated via novel object recognition (NOR) test (from day 24 to day 26 after FeCl3 injection) and Morris Water Maze (MWM) test (from day 29 to day 34 after FeCl3 injection). Additionally, on the day 3 and day 34 after FeCl3 injection, brain tissue samples were both harvested for biochemical detection or pathological analysis. isc, injection in the somatosensory cortex.
Figure 2. Fer-1 has an anticonvulsant effect against FeCl3-induced PTE. (A) Illustration of the injected site for Fer-1 and the location of electrode implantation for EEG recording; (B) Mean seizure scores after FeCl3 injection at the interval time of 90 min; (C) Representative EEG recordings and power spectrum density in the acute stage of PTE; (D) Quantification of EEG power spectra from day 1 to day 3 after FeCl3injection in different groups. The baseline EEG trace was monitored 30 min before FeCl3 injection; (E-F) Cumulative analysis of baseline power and total power; (G) Effects of Fer-1 on the epileptiform spike frequency (marked with spikes per 10 min) from day 1 to day 3 after FeCl3 injection in different groups; (H) Indication of heat map of seizure activity over time (per day) per mouse treated with either vehicle or Fer-1 in the acute stage of PTE; (I-K) Effects of Fer-1 on the seizure onset, number of seizures and time in seizure in FeCl3-induced PTE. Data were shown as mean ± SEM (n=6), *p<0.05 versus control group, **p<0.01 versus control group, ***p<0.001 versus control,#p<0.05 versus FeCl3+Vehicle group,##p<0.01 versus FeCl3+Vehicle group and###p<0.001 versus FeCl3+Vehicle group.