2.3. Racine score
The behavioral score was evaluated for 90 min according to Racing scale
(Racine et al., 1972) after FeCl3 injection. The
standards of seizure scoring were shown as we previously described (Li
et al., 2019): Stage 0, no response; stage 1, facial and whisker
rhythmic twitching; stage 2, head bobbing and circling; stage 3,
myoclonic and spasm in multiple limbs; stage 4, uncontrolled rearing and
falling; stage 5, general tonic-clonic seizures with running and jumping
and stage 6, death. Mice with stage 3 or higher were deemed to be
successfully induced. Throughout the seizure score assessment, two
participants were enrolled who were blind to the experimental design.