3.6. Fer-1 attenuates seizure-induced hippocampal damage in
FeCl3-induced PTE
Activation of ferroptosis process always triggers cell death (Ueda et
al., 2003). Thus, in our current work, Nissl staining was performed to
evaluate the effect of Fer-1 on neuron death following PTE. It was
evident that PTE in the acute stage (day 3 after FeCl3injection) induced enormous neuron death, especially in hippocampal DG
region and treatment with Fer-1 obviously improved cell viability
(Figure 7A, Figure 7B, Figure 7C and Figure 7D). Interestingly, in the
chronic stage of PTE (day 34 after FeCl3 injection),
Nissl staining results demonstrated that Fer-1 prevented neuron death in
hippocampal CA1 subregion of mice subjected to PTE. There was no
significant difference in the number of surviving neurons between each
group in CA3 and DG regions (Figure 7E, Figure 7F, Figure 7G and Figure
7H). Taken together, these findings implicate that Fer-1 ameliorates
hippocampal neuron death in FeCl3-induced PTE.