2.10. Statistical analysis
All experiments were carried out at least three independent replicates. The analyses and statistical significance were performed in a blinded manner and calculated using GraphPad Prism 8.0 software. The normal distribution of the data was evaluated with Shapiro-Wilk test. One-way ANOVA or RM-two-way ANOVA followed by Tukey’s tests, Sidak’s tests or unpaired t tests was used for multiple comparisons to normally distributed data. Non-normal data were analyzed by Kruskal-Wallis test or Mann Whitney U test. The details of statistical test for each experiment were summarized in Table 1. All results were presented as the mean ± SEM. P values of less than 0.05 were considered to indicate statistical significance.