2.10. Statistical analysis
All experiments were carried out at least three independent replicates.
The analyses and statistical significance were performed in a blinded
manner and calculated using GraphPad Prism 8.0 software. The normal
distribution of the data was evaluated with Shapiro-Wilk test. One-way
ANOVA or RM-two-way ANOVA followed by Tukey’s tests, Sidak’s tests or
unpaired t tests was used for multiple comparisons to normally
distributed data. Non-normal data were analyzed by Kruskal-Wallis test
or Mann Whitney U test. The details of statistical test for each
experiment were summarized in Table 1. All results were presented as the
mean ± SEM. P values of less than 0.05 were considered to indicate
statistical significance.