Total nucleic acid extraction and detection ofM.pneumoniae
Total nucleic acids were extracted using a throat swab extraction kit
(Health Gene Technologies, China) according to the manufacturer’s
instruction.M.
pneumonia was detected by SAT-MP kit (Shanghai Rendu Biotechnology Co,
Ltd). Primers were targeted to 16s
rRNA:MP-1(5’AATTTAATACGACTCACTATAGGGAGACACCGC
TCCACATGAAATTCCAAAACTCCC3’) and MP-2(5’CGGTAATACATAGGTCGCAAGC3’).The
probe is FAM-5’CGGACUAUUAAUCUAGAGUGUGUCCG3’-DABCYL. The detection limit
of this assay was 1000 DNA copies/ml regarding the respiratory
specimens.