Figure 3. Effect of eperisone on pre-developed pulmonary fibrosis.
Mice were treated with bleomycin (BLM, 1 mg/kg) or vehicle once only on day 0. The mice were then orally administered the indicated dose of eperisone (Epe) once daily for 9 days (from day 10 to day 18). Pulmonary tissue sections were prepared on day 20 and subjected to a histopathological examination (Masson’s trichrome staining; scale bar = 500 µm) (A). The collagen-positive area was determined based on Masson’s trichrome staining images (B). The pulmonary hydroxyproline level was determined on day 20 (C). The total respiratory system elastance, tissue elastance, and forced vital capacity (FVC) were measured on day 20 (D). Values represent the mean ± SEM **P < 0.01 ; *P < 0.05 ; NS, not significant.