Treatment of mice with BLM, eperisone, and other reagents
Mice were anesthetized with isoflurane and intratracheally administered
BLM (1 mg/kg, once) in sterile saline via a single channel pipette
(P200). Ten days after BLM administration, eperisone (15 or 50 mg/kg),
tolperisone (15 mg/kg), pirfenidone (200 mg/kg), and nintedanib (30
mg/kg) were administered orally for a total of 9 days from day 10 to day
18. Various analyses were then performed on day 20.
In the adverse effect study, 10 days after BLM administration, 250 mg/kg
of eperisone was orally administered once, which was five times the dose
that showed efficacy. Twenty-four hours after eperisone administration,
the fecal condition of the mice was visually examined. In addition,
plasma samples and stomach and colon tissues were collected from the
mice. Analysis of the plasma samples was performed by TRANS GENIC INC.
(https://www.transgenic.co.jp/).