2.8 Detection of volatile components in wheat seedlings.
Compared with other groups, the combination of nano-Se (5mg/L) and MT (10mg/L) showed a better effect on reducing of aphid number. Gas Chromatography-Ion Mobility Spectrometer (GC-IMS) was used to analyze VOCs released from the wheat seedlings. About 0.5g wheat seedlings samples were weighted in a 20-mL headspace vial. The vials were equilibrated at 60℃ for 20 min before headspace injection. Table S4 (the analysis conditions of GC-IMS unit) and Table S5 (gas chromatographic conditions) have specific parameters. Wheat seedlings samples were analyzed used with VOCAL and three plugins (Reporter, Gallery Plot, and Dynamic PCA), which can qualitative and quantitative the data and spectrogram.