2.1 Plant growth.
The wheat plants used in the experiments were grown in the intelligent
greenhouse of the College of Agriculture, China Agricultural University
(Beijing, China). Wheat seeds (Liaochun10) were soaked in 0.5% sodium
hypochlorite for 30 min and then cleaned five times with distilled
water. The wheat seeds were planted in plastic pots (14 cm in diameter
and 11 cm in height) containing organic soil (nutritional soil: peat:
vermiculite = 4:1:1). During the whole experiment period, the plants
maintained the relative humidity at 70% at the day/night temperature of
25/20 ℃ with a day/night cycle of 14/10 h and the light intensity at
80µmol m-2 s-1. The nano-Se (50-78
nm) applied in this study was characterized and reported in our previous
study
(Dong Li, An, Wu, Li, & Pan, 2020). The plant experiment was separated
into four groups including three levels of nano-Se treatment (5, 10, and
20mg/L) and the controls. These four treatments each had five
duplicates.