3.2 Effects of different concentrations of nano-Se on phytohormone in wheat seedlings.
As shown in Figure 2A-D, nano-Se application (5.0, 10.0, and 20.0 mg/L) increases the JA, MT, and DIMBOA content, but reduce the SA content in wheat. Compared with the control, nano-Se at the application rate of 5.0 mg/L could enhance the JA, MT and DIMBOA concentration by 150%, 88.6%, and 64.3%; at the same time, SA concentration decreased by 88.0%.
3.3 Antioxidant capacity of wheat seedlings with nano-Se treated.
The application of nano-Se can alter the antioxidant capacity of wheat seedlings as measured by antioxidant enzymes and some related chemicals (Figure 3). Among the three levels of nano-Se treatments, the concentration at 5.0 mg/L manifested the greatest impacts. Antioxidant enzyme activity APX, LOX, GSH-Px, CAT, and POD increased by 410.9, 306.9, 69.2, 38.5, and 10.7%, respectively. The concentration of GSH, Pro, and vitamin E (VE) contents enhanced by 45.9%, 19.0%, and 44.2%, respectively (nano-Se 5 mg/L). As for MDA (Figure 3H), the concentration decreased by 13.6%. Nano-Se (5.0 mg/L) could significantly (P <0.05) improve the antioxidant capacity of wheat seedlings by increasing the activity of antioxidant enzymes and the related chemical concentration.