Nanoselenium enhanced wheat resistance to aphids by regulating biosynthesis of DIMBOA and volatile components
Chunran Zhou a, 1, Dong Li a, 1, Xinlei Shi a, Jingbang Zhang a, Quanshun An a, Yangliu Wu a, Lu Kanga, Jia-Qi Li a and Canping Pana, *
a Innovation Center of Pesticide Research, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China Yuanmingyuan West Road 2, Beijing 100193, PR China
*Corresponding author
Canping Pan: canpingp@cau.edu.cn
1 Chunran Zhou and Dong Li contributed equally to this paper.
Abstract: The mechanism of nanoselenium (nano-Se) improving plant components induced resistance to aphids is unkonwn. . In this study, nano-Se (5.0 mg/L) foliar sprayed could significantly reduceSitobion avenae number (36%) compared with the control. Foliar application of nano-Se enhances the antioxidant capacity by reducing MDA and increasing GSH-Px, CAT, GSH, Pro and VE concentration in wheat seedlings. Phenylpropane pathway was activated by nano-Se bio-fortification, which increased apigenin and caffeic acid concentrations. The high-level expression of the related genes (TaBx1A , TaBx3A , TaBx4A, TaASMT2, andTaCOMT ) induced the promotion of melatonin (88.6%) and DIMBOA (64.3%). Different ratios of the secondary metabolites to nano-Se were conducted to examine the effects on wheat resistance to theSitobion avenae . The results revealed the combination of nano-Se and melatonin can achieve the best overall performance by reducing theSitobion avenae number by 52.2%. The study suggest that the coordinative applications of nano-Se and melatonin could be more effectively improve the wheat resistance to aphids via promotion of volatile organic compound synthesis and modulation in phenylpropane and indole metabolism pathways.
KEYWORDS: wheat, aphids, plant resistance, nanoselenium, metabolite pathway.