As part of ongoing surveillance of carbapenemase producing Enterobacteriaceae (CPE), bacterial isolates were sent to the Microbiological Diagnostic Unit (MDU) from hospitals statewide in Victoria, Australia.  After culture on non-selective media, single colonies were picked and DNA extracted using MoBio powersoil kits.  PCR of 587 bp of the blaIMP-4 gene was performed using primers IMP-A 5'-GAA GGY GTT TAT GTT CAT AC-3' and IMP-B 5'-GTA MGT TTC AAG AGT GAT GC-3'.   MALDI-ToF (Biomireux) was used for species identification.