Svep1+/- Mouse Studies
Animal experimentation was approved by the local animal ethics committee and performed according to ARRIVE (Animal Research: Reporting of In Vivo Experiments) guidelines and under United Kingdom Home Office Project Licence (P4E9A1CCA). All mice were housed in a specific pathogen-free facility in an individually ventilated caging system. Mice were group housed wherever possible, and their health status was checked routinely. No mice demonstrated any adverse effects. C57BL/6J mice were purchased originally from Charles River, then bred in the Preclinical Research Facility in the University of Leicester, to provide animals for the study. Genetically altered animals, B6N(Cg)-Svep1 tm1b(EUCOMM)Hmgu/J (reporter-tagged deletion allele, https://www.informatics.jax.org/allele/MGI:5509058;Svep1 +/-) was purchased from the Jackson Laboratory (Bar Harbor, ME, USA). 8-24 week old mice of both genders were used in experiments.