Flow cytometry
iVSMCs were dissociated using Accutase. CD140+staining was quantified using single cell suspensions incubated using an
APC-direct labelled antibody diluted in flow buffer (BSA (0.5%), EDTA
(2 mM), PBS, pH 7.2). Samples were run on a Beckman Coulter Gallios flow
cytometer and analysed using Kaluza flow cytometry analysis software
(Beckman Coulter).