Svep1+/- Mouse Studies
Animal experimentation was approved by the local animal ethics committee
and performed according to ARRIVE (Animal Research: Reporting of In Vivo
Experiments) guidelines and under United Kingdom Home Office Project
Licence (P4E9A1CCA). All mice were housed in a specific pathogen-free
facility in an individually ventilated caging system. Mice were group
housed wherever possible, and their health status was checked routinely.
No mice demonstrated any adverse effects. C57BL/6J mice were purchased
originally from Charles River, then bred in the Preclinical Research
Facility in the University of Leicester, to provide animals for the
study. Genetically altered animals,
B6N(Cg)-Svep1 tm1b(EUCOMM)Hmgu/J
(reporter-tagged deletion allele,
https://www.informatics.jax.org/allele/MGI:5509058;Svep1 +/-) was purchased from the Jackson
Laboratory (Bar Harbor, ME, USA). 8-24 week old mice of both genders
were used in experiments.