Flow cytometry
iVSMCs were dissociated using Accutase. CD140+staining was quantified using single cell suspensions incubated using an APC-direct labelled antibody diluted in flow buffer (BSA (0.5%), EDTA (2 mM), PBS, pH 7.2). Samples were run on a Beckman Coulter Gallios flow cytometer and analysed using Kaluza flow cytometry analysis software (Beckman Coulter).