Key Results
We confirmed the ligation of SVEP1 to integrin α9β1 and additionally
found SVEP1 to directly bind to integrin α4β1. Inhibition of SVEP1,
integrin α4β1 or α9β1 significantly enhanced
[Ca2+]i release in isolated VSMCs
to Gαq/11-vasoconstrictors. This response was confirmed
in whole vessels where a greater contraction to U46619 was seen in
vessels from Svep1+/- mice compared to
littermate controls or when integrin α4β1 or α9β1 were inhibited.
Inhibition studies suggested that this effect was mediated via VGCCs in
a PKC dependent mechanism.