Cell transfection
iVSMCs were transiently transfected with non-targeting control (Qiagen
siRNA, cat: 1022076), ITGA9 (Qiagen FlexiTube siRNA, cat: S100034272),
SVEP1 (ThermoFisher Scientific Stealth siRNA, cat: 1299001) or ITGA4
(Dharmacon SMARTpool of 4 siRNAs, cat: SO-2757075G) using Lipofectamine
RNAiMAX (Thermofisher) diluted in OptiMEM in SMGM2. Media was changed
after 24 hours, with cells used at 48 hours.