Western blotting
Cells were lysed in modified RIPA buffer (Tris HCl (50 mM), EDTA (1 mM), Halt Protease Inhibitor cocktail (Thermofisher), pH7.4. Western Blot Analysis Protein content was measured using the Novex® protein separation kit (Thermofisher). Equal amounts of protein lysates were separated by SDS-PAGE before blotting onto nitrocellulose membrane. Membranes were probed with primary antibodies (see Table S2), detected with horseradish peroxidase conjugated secondary antibodies and visualised by enhanced chemiluminescence (GE Healthcare). Quantitative signals were derived by densiometric analysis using ImageQuant™ TL on an ImageQuant™ LAS 4000 Luminescent Image Analyzer (Fujifilm).