Figure 5. ILDR1 Expression after swine influenza virus
infection. (A) The lungs of mice were taken after infection 0, 3 and14
days for immunohistochemical detection. ILDR1 was detected by
immunohistology using rabbit anti-ILDR1, visualized with
3,3,-diaminobenzidine and counterstained with
hematoxylin. Scale bar: 20 μm. The expression levels of the ILDR1 after
infection relative to that of normal expression were analyzed by areal
density (B).(C) HEK 293T cells were infected with different
doses(MOI=0,0.01,0.1,1,10) for 48h , and supernatants were collected,
then the expression of ILDR1 was confirmed by quantitative
reverse-transcription PCR. Data represent the mean value±SD. Asterisks
indicate statistical difference (Unpaired t test; *P<0.05,
**P<0.01, ***P<0.001). (D) Expression ofILDR1 in virus-infected cells at an MOI of 0.1. The supernatants
were collected at different time points (0h, 6h, 12h, 24h, 48h) and
subjected to RT-qPCR. Data represent the mean value±SD. Asterisks
indicate statistical difference (Unpaired t test; *P<0.05,
**P<0.01, ***P<0.001). (E) Virus replication in
ILDR1-overexpressing HEK293T cells. Cells were transduced with ILDR1-GFP
or pEGFPN2 for 24h, and then infected with SIV at an MOI of 0.1.
Supernatants were collected at the indicated timepoints, and virus
titers or NP expression were determined by TCID50, CCK-8 (F) and RT-qPCR
(G).