Figure 5. ILDR1 Expression after swine influenza virus infection. (A) The lungs of mice were taken after infection 0, 3 and14 days for immunohistochemical detection. ILDR1 was detected by immunohistology using rabbit anti-ILDR1, visualized with 3,3,-diaminobenzidine and counterstained with hematoxylin. Scale bar: 20 μm. The expression levels of the ILDR1 after infection relative to that of normal expression were analyzed by areal density (B).(C) HEK 293T cells were infected with different doses(MOI=0,0.01,0.1,1,10) for 48h , and supernatants were collected, then the expression of ILDR1 was confirmed by quantitative reverse-transcription PCR. Data represent the mean value±SD. Asterisks indicate statistical difference (Unpaired t test; *P<0.05, **P<0.01, ***P<0.001). (D) Expression ofILDR1 in virus-infected cells at an MOI of 0.1. The supernatants were collected at different time points (0h, 6h, 12h, 24h, 48h) and subjected to RT-qPCR. Data represent the mean value±SD. Asterisks indicate statistical difference (Unpaired t test; *P<0.05, **P<0.01, ***P<0.001). (E) Virus replication in ILDR1-overexpressing HEK293T cells. Cells were transduced with ILDR1-GFP or pEGFPN2 for 24h, and then infected with SIV at an MOI of 0.1. Supernatants were collected at the indicated timepoints, and virus titers or NP expression were determined by TCID50, CCK-8 (F) and RT-qPCR (G).