3.1 FFA improved survival and neurologic outcome after CA/CPR
A total of 200 mice were used for the study (Fig. 1B). Among them, 180
mice underwent CA/CPR and 20 mice received sham operation. Of the 153
mice that successfully resuscitated, 29 mice died before the settled
time points were excluded. There was no difference in body weight, time
required for ROSC, total epinephrine dose, and heart rate
among
the experimental groups (Table S1, S2). The partial pressure of oxygen
(PaO2) and oxygen saturation (SaO2) of arterial blood samples obtained
at 2 h after CPR did not differ among the experimental groups (data not
shown). There was no difference in core body temperature among the
experimental groups for 1 h after CA/CPR (data not shown).
We firstly observed the survival rate during 7 days after CA/CPR and the
results revealed that 35% mice (7 of 20) survived in the vehicle group
compared with 65% mice (13 of 20) in the FFA group until day 7 at the
end of the study (P < 0.05) (Fig. 2A). Furthermore, we
used a 10-point scoring system to assess the overall neurologic deficits
and found that FFA- treated mice displayed significant better
performance on this test at 3 days after ROSC (Fig. 2B). Nonetheless, no
apparent difference was found in the neurologic score at 7 days between
the vehicle and FFA group, possibly because the animals with more severe
neurological deficits in the vehicle group had died. Collectively, these
data indicated that post-CA treatment with FFA resulted in better 7-day
survival and functional outcome.
3.2 FFA alleviated neuronal loss and suppressed activation of
glial
cells