3.3 FFA mitigated CA/CPR-induced neuronal degeneration and cell apoptosis in the brain
To validate whether FFA treatment could ameliorate neuronal degeneration, we carried out FJC staining to confirm the degeneration of neurons at 3 days after CA/CPR. Results revealed that CA/CPR induced an extensive degenerative reaction in the cortex, striatum, and hippocampus, which was partly preserved by FFA treatment (Fig. 4A, C). We proceeded to evaluate the cellular apoptosis in the brain at 3 days after CA/CPR among different groups. TUNEL staining was employed to examine cell apoptosis. Compared with the sham group, robust cellular apoptosis was detected in the cortex, striatum, and hippocampus, whereas FFA treatment could decreased TUNEL-positive cells (Fig. 4B, D). In summary, these findings suggested that FFA treatment suppressed neuronal degeneration and cell death in the brain of mice subjected to CA/CPR.