Figure 10. Effects of FFA on the integrity of blood-brain
barrier and microglia/macrophages polarization inTrpm4−/− mice after cardiac arrest and
cardiopulmonary resuscitation.
Representative photomicrographs of immunofluoresence staining with IgG
antibody (red) from each group at 3 days after return of spontaneous
circulation. Scale bar, 100 μm or 50 μm. (B )
Semi-quantitative results of IgG leakage. (C ) Representative
photomicrographs of immunofluorescence staining for CD16/32 (green),
Arg1 (green) and Iba1 (red) in the brain of each group at 3 days after
return of spontaneous circulation. Scale bar, 50 μm. (D )
Quantification of the percentage of CD16/32 and Iba1 double-positive
cells as well as Arg1 and Iba1 double-positive cells.
*P <0.05, **P <0.01,
***P <0.001 versus the WT group. Iba1: ionized
calcium-binding adapter molecule 1; KO: vehicle-treatedTrpm4−/− mice; KO (FFA): flufenamic
acid-treated Trpm4−/− mice; WT:
vehicle-treated wild-type mice.