3.3 FFA mitigated CA/CPR-induced neuronal degeneration and cell
apoptosis in the brain
To validate whether FFA treatment could ameliorate neuronal
degeneration, we carried out FJC staining to confirm the degeneration of
neurons at 3 days after CA/CPR. Results revealed that CA/CPR induced an
extensive degenerative reaction
in
the cortex, striatum, and hippocampus, which was partly preserved by FFA
treatment (Fig. 4A, C). We proceeded to evaluate the cellular apoptosis
in the brain at 3 days after CA/CPR among different groups. TUNEL
staining was employed to examine cell apoptosis. Compared with the sham
group, robust cellular apoptosis was detected in the cortex, striatum,
and hippocampus, whereas FFA treatment could decreased TUNEL-positive
cells (Fig. 4B, D). In summary, these findings suggested that FFA
treatment suppressed neuronal degeneration and cell death in the brain
of mice subjected to CA/CPR.