2.9 MRI
MRI was performed at 72 h after ROSC as described before [31]. During MRI, mice were anesthetized and maintained with inhaled isoflurane (5% induction, 1–2% maintenance). The brains were scanned from the olfactory bulb to the brainstem by a 7.0 Tesla horizontal-bore magnet (PharmaScan 70/16 US, Bruker). Diffusion-weighted imaging (DWI) method sequence was obtained. To quantify brain edema after CA/CPR, average ADC values in lateral hippocampus, striatum and cortex were calculated by a researcher blinded to group allocation.