2.4 Survival study and neurologic outcome assessment
Survival was recorded daily for 7 days after CA/CPR. A previously reported neurological function scoring system was utilized to assess the neurologic deficiency at day 3 and day 7 after ROSC in all successfully resuscitated animals, which was conducted by two investigators who were masked to animal grouping [29]. The scoring system consisted of five parameters: consciousness, corneal reflex, respiration, coordination, and movement/activity. A total score of 10 was considered as normal.