Figure 3. Effects of FFA on neuronal loss and activation of microglia and astrocytes after cardiac arrest and cardiopulmonary resuscitation.
(A ) Representative photomicrographs of post-cardiac arrest neuropathological damages characterized by immunofluorescence staining for NeuN, MAP2, Iba1, and GFAP in the hippocampal CA1 region of the sham group and the experimental groups at 7 days after return of spontaneous circulation. Scale bar, 100 μm or 50 μm. (B ) Semi-quantitative results of NeuN, MAP2, Iba1, and GFAP. ***P <0.001 versus the sham group; ##P <0.01, ###P <0.001 versus the vehicle group. FFA: flufenamic acid; GFAP: glial fibrillary acidic protein; Iba1: ionized calcium-binding adapter molecule 1; MAP2: microtubule-associated protein 2; NeuN: neuronal nucle.