2.9 MRI
MRI was performed at 72 h after ROSC as described before [31].
During MRI, mice were anesthetized and maintained with inhaled isoflurane
(5% induction, 1–2% maintenance). The brains were scanned from the
olfactory bulb to the brainstem by a 7.0 Tesla horizontal-bore magnet
(PharmaScan 70/16 US, Bruker). Diffusion-weighted imaging (DWI) method
sequence was obtained. To quantify brain edema after CA/CPR, average ADC
values in lateral hippocampus, striatum and cortex were calculated by a
researcher blinded to group allocation.