FIGURE 1 Breeding range (darker shaded area) of black-throated green warbler (Setophaga virens virens ) and putative subspecies, Wayne’s warbler (S. v. waynei ). Study sites are indicated with black symbols where S. v. virens were sampled and yellow symbols where S. v. waynei were sampled. NF = National Forest, WMA = Wildlife Management Area, SF = State Forest.
FIGURE 2 Principal components analysis (PCA) differentiating populations across the Setophaga virens North American breeding range. All populations are S. v. virens except for those individuals from North Carolina (NC) identified as S. v. waynei .
FIGURE 3 Windowed F ST estimates (10kb) comparing the genomes of Setophaga virens virens and S. v. waynei(A), including two regions—one on chromosome 6 (B) and one on the Z (sex) chromosome (C)—with elevated differentiation above the background.
FIGURE 4 Detail view of regions of elevated divergence comparing the genomes of Setophaga virens virens and S. v. waynei. Grey points illustrate F ST estimates for 10kb windows. Variable colors indicate different annotated genes in these regions on (a) chromosome 6 (five annotated genes) and (b) the Z chromosome (four annotated genes and one gene of unknown function).
FIGURE 5 Bootstrap phylogeny from ngsDist based on genome-wide genotype likelihoods arcoss all Setophaga virens samples and one outgroup (S. chrysoparia ).
FIGURE S1 Detail view of narrowed windowedF ST estimates (2 bp) comparing the genomes ofSetophaga virens virens and S. v. waynei for two regions (A) on chromosome 6 (B) the Z chromosome with elevated differentiation above the background. The red line indicates F ST of 0.7.