FIGURE 1 Breeding range (darker shaded area) of black-throated
green warbler (Setophaga virens virens ) and putative subspecies,
Wayne’s warbler (S. v. waynei ). Study sites are indicated with
black symbols where S. v. virens were sampled and yellow symbols
where S. v. waynei were sampled. NF = National Forest, WMA =
Wildlife Management Area, SF = State Forest.
FIGURE 2 Principal components analysis (PCA) differentiating
populations across the Setophaga virens North American breeding
range. All populations are S. v. virens except for those
individuals from North Carolina (NC) identified as S. v. waynei .
FIGURE 3 Windowed F ST estimates (10kb)
comparing the genomes of Setophaga virens virens and S. v. waynei(A), including two regions—one on chromosome 6 (B) and one on the Z
(sex) chromosome (C)—with elevated differentiation above the
background.
FIGURE 4 Detail view of regions of elevated divergence
comparing the genomes of Setophaga virens virens and S. v.
waynei. Grey points illustrate F ST estimates for
10kb windows. Variable colors indicate different annotated genes in
these regions on (a) chromosome 6 (five annotated genes) and (b) the Z
chromosome (four annotated genes and one gene of unknown function).
FIGURE 5 Bootstrap phylogeny from ngsDist based on genome-wide
genotype likelihoods arcoss all Setophaga virens samples and one
outgroup (S. chrysoparia ).
FIGURE S1 Detail view of narrowed windowedF ST estimates (2 bp) comparing the genomes ofSetophaga virens virens and S. v. waynei for two regions (A) on
chromosome 6 (B) the Z chromosome with elevated differentiation above
the background. The red line indicates F ST of
0.7.