Figure Legends
Figure 1 Analysis of uc.375 expression characteristics. A. Scatter analysis chart of lncRNAs expression in hyperoxia-induced BPD lung tissue and normal lung tissue. B. Cluster analysis. C-F. uc.375 Bioinformatics characteristics. G. The relative mRNA expression level of uc.375 was detected by realtime PCR in lung tissues at different time points (D0, D1, D3, D5, D7 days) during BPD modeling. H. The localization of uc.375 in MLE 12 cells.
Figure 2 The effect of uc.375 silence on MLE 12 cells function and FoxA1 expression. A. The proliferation activity of MLE 12 cells was detected by CCK-8 assay. B. The cell apoptosis was determined by Hoechst/PI staining in MLE12 cells. C. The protein levels of Cleaved-caspase3, FoxA1, SP-C and UCP2 were investigated by western blot in MLE12 cells. D. The cell apoptosis was determined using low cytometry assay in MLE12 cells. E-H. The relative mRNA expression levels of uc.375, FoxA1, SP-C and UCP2 were detected by realtime PCR in MLE12 cells. *P < 0.05, **P < 0.01vs . sh-Con.
Figure 3 The effect of uc.375 overexpression on the function of MLE 12 cells. A. The proliferation activity of MLE 12 cells was detected by CCK-8 assay. B. The cell apoptosis was determined by Hoechst/PI staining in MLE12 cells. C. The protein levels of caspase3, FoxA1, SP-C and UCP2 were investigated by western blot in MLE12 cells. D. The cell apoptosis was determined using low cytometry assay in MLE12 cells. E-H. The relative mRNA expression levels of uc.375, FoxA1, SP-C and UCP2 were detected by realtime PCR in MLE12 cells. *P< 0.05, **P < 0.01 vs . sh-Con.
Figure 4 Effects of uc.375 silencing and FoxA1 silencing on the function of MLE 12 cells. A. The proliferation activity of MLE 12 cells was detected by CCK-8 assay. B. The cell apoptosis was determined by Hoechst/PI staining in MLE12 cells. C. The protein levels of caspase3, FoxA1, SP-C and UCP2 were investigated by western blot in MLE12 cells. D. The cell apoptosis was determined using low cytometry assay in MLE12 cells. E-H. The relative mRNA expression levels of uc.375, FoxA1, SP-C and UCP2 were detected by realtime PCR in MLE12 cells. *P < 0.05, **P < 0.01 vs. sh-Con.
Figure 5 Effects of uc.375 overexpression and FoxA1 overexpression on the function of MLE 12 cells. A. The proliferation activity of MLE 12 cells was detected by CCK-8 assay. B. The cell apoptosis was determined by Hoechst/PI staining in MLE12 cells. C. The protein levels of caspase3, FoxA1, SP-C and UCP2 were investigated by western blot in MLE12 cells. D. The cell apoptosis was determined using low cytometry assay in MLE12 cells. E-H. The relative mRNA expression levels of uc.375, FoxA1, SP-C and UCP2 were detected by realtime PCR in MLE12 cells. *P < 0.05, **P < 0.01 vs. sh-Con.