2.6 Flow cytometry assay
The apoptosis rate of MLE12 cells was detected by Annexin V-FITC/PI
Apoptosis Detection Kit (Yeasen, Shanghai, China) via flow cytometry.
After cultured for 48 hours, cells were collected, following by staining
with 10 μL of Annexin V-FITC and PI. Then cells were analyzed by flow
cytometer (BD, New Jersey, USA) 17.