3.3 The effect of uc.375 overexpression on the function of MLE 12 cells
To further assess the effect of uc.375 overexpression on MLE 12 cells function, MLE 12 cells were stably transfected with uc.375 overexpression lentiviral vector to overexpress uc.375. CCK-8 assay was conducted and cell viability was increased by uc.375 overexpression (Figure 3A). In addition, the effect of uc.375 overexpression on cell apoptosis was assessed by Hoechst/PI staining and flow cytometry. The cell apoptosis was remarkably reduced by uc.375 overexpression (Figure 3B and 3D). Moreover, the protein levels of caspase3, FoxA1, SP-C and UCP2 were investigated by western blot in MLE12 cells. The results showed that SP-C and UCP2 were increased, whereas caspase3 and FoxA1 were markedly inhibited by uc.375 overexpression (Figure 3C). In addition, the qRT-PCR results confirmed that uc.375 level was significantly elevated by uc.375 overexpression, the SP-C and UCP2 mRNA expression were stimulated, whereas FoxA1 was markedly suppressed by uc.375 overexpression (Figure 3E-H). Taken together, uc.375 overexpression promoted cell proliferation and repressed apoptosis.