2.6 Flow cytometry assay
The apoptosis rate of MLE12 cells was detected by Annexin V-FITC/PI Apoptosis Detection Kit (Yeasen, Shanghai, China) via flow cytometry. After cultured for 48 hours, cells were collected, following by staining with 10 μL of Annexin V-FITC and PI. Then cells were analyzed by flow cytometer (BD, New Jersey, USA) 17.