2.4 Hoechst PI staining
The single cell suspension was prepared routinely, the final concentration of Hoechst 33258 was 1 MG/ML, the final concentration of Hoechst 33258 was dissolved in water at 37 °C for 7 minutes, the dye solution was cooled on ice, the dye solution was centrifuged and PBS was overhung, the final concentration of PI solution was 5 MG/ML, the ice bath was added, the dye solution was centrifuged and PBS was washed once, the Blue fluorescence at 400 ~ 500 nm and the red fluorescence at 630 nm or above were recorded by direct external laser Flow cytometry analysis.