Figure 6. IL-25 promotes antigen uptake by BmEOS.
(A) Flowchart of isolation and culture of BmEOS.
(B-D) Identification of BmEOS. Histogram of Siglec-F expression at indicated times of mouse BmEOS culture(B). Flow cytometry quantification of Siglec-F positive BmEOS using isotype control and anti-Siglec-F AF647-conjugated antibody(C). Light microscopic image of BmEOS at culture day 11, Diff-Quick stain(D).
(E) At day 10~13, BmEOS were treated with or without HDM in the absence or presence of IL-25 for 48 h. During the last 12 hours, DQ-OVA was added. The percentage of DQ-OVA+ BmEOS and MFI of DQ-OVA were analyzed by flow cytometry. Representative graphs, statistical data and MFI values are shown. Cells with DQ-OVA incubated at 4℃ were set as isotype.
n=4, Unpaired Student’s t test (E) , *p<0.05, **p<0.01