IL-25 promoted antigen uptake by bone marrow-derived eosinophils
in vitro
Considering the predominant role for antigen uptake in APC, we looked at
the capacity of BmEOS to take up HDM on exposure to IL-25. We generated
eosinophils from bone marrow of mice (Figure 6A) and identified that
BmEOS become mature and reached at high purity after day 10 (Figure
6B~D). To assess whether IL-25 modulated the ability of
eosinophils to uptake antigen directly, we treated BmEOS with DQ-OVA
when pulsed with or without HDM. When pulsed with HDM, the uptake
ability of BmEOS slightly raised but without significant statistical
difference compared with control group. Pulsed with HDM and IL-25
increased significantly uptake of DQ-OVA by BmEOS, as compared to pulsed
with HDM alone (Figure 6E). These data suggested that IL-25 promoted the
ability of eosinophils to take up the antigen.