IL-25 promoted antigen uptake by bone marrow-derived eosinophils in vitro
Considering the predominant role for antigen uptake in APC, we looked at the capacity of BmEOS to take up HDM on exposure to IL-25. We generated eosinophils from bone marrow of mice (Figure 6A) and identified that BmEOS become mature and reached at high purity after day 10 (Figure 6B~D). To assess whether IL-25 modulated the ability of eosinophils to uptake antigen directly, we treated BmEOS with DQ-OVA when pulsed with or without HDM. When pulsed with HDM, the uptake ability of BmEOS slightly raised but without significant statistical difference compared with control group. Pulsed with HDM and IL-25 increased significantly uptake of DQ-OVA by BmEOS, as compared to pulsed with HDM alone (Figure 6E). These data suggested that IL-25 promoted the ability of eosinophils to take up the antigen.