Figure 7. IL-25-activated Eosinophils promote Th2 polarization in vitro.
(A) Schematic diagram of isolated naïve CD4+ T cells from mice and co-culture of T-BmEOS system.
(B) Flow cytometry identification of isolated naïve CD4+T cells (viable CD45+CD3+CD4+ CD62L+CD44-).
(C-D) Purified naïve CD4+ T cells and BmEOS were co-cultured for 96 h with HDM in the presence or absence of IL-25. Naïve CD4+ T cells alone were also cultured. The differentiation of T subsets was detected by flow cytometry.
n=3, Unpaired Student’s t test (C,D) , *p<0.05.