Figure 6. IL-25 promotes antigen uptake by BmEOS.
(A) Flowchart of isolation and culture of BmEOS.
(B-D) Identification of BmEOS. Histogram of Siglec-F expression at
indicated times of mouse BmEOS culture(B). Flow cytometry quantification
of Siglec-F positive BmEOS using isotype control and anti-Siglec-F
AF647-conjugated antibody(C). Light microscopic image of BmEOS at
culture day 11, Diff-Quick stain(D).
(E) At day 10~13, BmEOS were treated with or without HDM
in the absence or presence of IL-25 for 48 h. During the last 12 hours,
DQ-OVA was added. The percentage of DQ-OVA+ BmEOS and MFI of DQ-OVA were
analyzed by flow cytometry. Representative graphs, statistical data and
MFI values are shown. Cells with DQ-OVA incubated at 4℃ were set as
isotype.
n=4, Unpaired Student’s t test (E) , *p<0.05, **p<0.01