IL-25 exposure induced eosinophil recruitment in vivo
IL-25 was shown in previous research to directly activate conventional DCs in vivo23. Thus, we sought to determine the effect of IL-25 in vivo on eosinophils. We administered recombinant murine IL-25 (1μg/mouse) or PBS to WT mice. After 24 h, lungs were digested and analyzed for infiltrated lung cell differentiation (Figure 3A). There were significantly more eosinophils in lungs of IL-25-treated mice compared with PBS-control mice (Figure 3B,3C). Thus, IL-25 promotes eosinophils to recruit to lungs in vivo.