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\begin{document}

\title{Construction and characterization of a full-length cDNA infectious clone
of emerging porcine Teschovirus-2}



\author[1]{Yuying Li}%
\author[2]{Shengnan Chen}%
\author[3]{Yaokai Shi}%
\author[1]{Haixin Huang}%
\author[1]{Wei Wang}%
\author[4]{Min Zheng}%
\author[1]{Chenchen Zhao}%
\author[1]{Xinyu Zhang}%
\author[1]{Xiaoxiao Lei}%
\author[1]{chao sun}%
\author[2]{Hao Liu}%
\author[1]{Tian Lan}%
\affil[1]{Wenzhou University}%
\affil[2]{Foshan University}%
\affil[3]{Wenzhou Medical University}%
\affil[4]{Guangxi Centre for Animal Disease Control and Prevention Nanning 530001 China}%


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  \date{May 26, 2024}


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\begin{abstract}
\emph{Porcine teschovirus} (PTV) is a causative agent of polio,
encephalomyelitis, reproductive disorders, and gastrointestinal and
respiratory diseasesin swine. In this study, the PTV-2 GX/2020 strain
was isolated from pig intestinal tissue using ST cells. Phylogenetic
analysis of VP1 nucleotide sequences indicated that the GX/2020 strain
was closely related to PTV-2 (86\%). Furthermore, a full-length cDNA
infectious clone of GX/2020 was constructed using seamless ligation
technology. The rescued virus was had a genome sequence largely
consistent with the parental virus sequence and had similar growth
characteristics. Overall, the PTV-2 virus successfully isolated in this
study and the employed reverse genetic platform have laid a foundation
for studying the pathogenic mechanism of porcine Teschovirus.%
\end{abstract}%



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