Expression of candidate miRNA from literature
To compare our results to previously published data sets, we examined
expression of previously reported miRNAs involved in diapause
regulation, of which only 50% could be identified in P. napi(Table S7). Of these eight identified miRNAs (miR-9c-5p, miR-13b-3p,
miR-14-5p, miR-31-5p, miR-92-3p, miR-275-3p, miR-282-5p, and bantam-5p),
all of them were found to be differentially expressed during diapause in
the head tissue (Figure 7), with all falling in different clusters
except miR-92-3p and bantam-5p both being in cluster H4. In the abdomen
tissue, six were differentially expressed during diapause (miR-13b-3p,
miR-14-5p, miR-31-5p, miR-92-3p, miR-282-5p, and bantam-5p) and only 2
(miR-31-5p and miR-282-5p) found in any identified clusters. Only two
were differentially expressed during diapause termination, miR-14-5p and
miR-92-3p, with miR-92-3p being only significant in the head, and
miR-14-5p in both.