Trial 2: Effect of MP-010 on survival, behavioral tests, and genetic markers
This trial was conducted at the animal facility of the University of Zaragoza using the colony of SOD1G93A mice that has been bred for years by Dr. Osta’s group. MP-010 was given to 21 SOD1G93A mice (n=8/13 females/males) in the drinking water at a concentration of 1 mM, treating the mice at a dose of 61 mg/kg. Simultaneously, another group of 20 SOD1G93A mice (n=9/11 females/males) was treated with vehicle as the control group. The treatment started at 8 weeks of age and was weekly renewed until each mouse reached its respective humane endpoint. Throughout the treatment, all mice underwent weekly weight measurements and motor behavioral tests, including rotarod and hanging-wire tests. All behavioral experiments in the three trials were conducted under blind conditions regarding the treatment.
Trial 3: Effect of MP-001 on survival, behavioral tests and genetic markers
This trial was conducted at the animal facility of the University of Zaragoza using the colony of SOD1G93A mice that has been bred for years by Dr. Osta’s group. This experimental study included one group of 19 SOD1G93A mice (n=9/10 females/males) to which compound MP-001 was administered in the drinking water at a concentration of 0.85 mM, resulting in 61 mg/kg dose, and another similar group of 17 SOD1G93A mice (n=9/8 females/males) treated with vehicle as control. All mice underwent weekly weight measurements and behavioral tests, including rotarod and hanging-wire tests, and the survival time was monitored.