Statistical methods
Data are shown as mean ± SEM. Electrophysiological and functional
measurements were analyzed with Two-way ANOVA, applying Bonferroni
post-hoc test for multiple comparisons. Histological data were analyzed
using One-way ANOVA with Bonferroni multiple comparisons test. The
survival time of mice was evaluated using Kaplan-Meier analysis and
Log-Rank test. Comparisons of means of gene and protein expression were
analyzed by unrelated t-test. The computer-assisted analysis of the
bands obtained in western blot was performed with AlphaEase FC software
(Bonsai Technologies Group, S.A.). Statistical analysis was performed
using SPSS (version 20, IBM, Armonk, NY, USA), and graphs were made
using GraphPad Prism Software (version 5, La Jolla, CA, USA) and
Microsoft Excel. Statistical significance was set at p<0.05.