Trial 2: Effect of MP-010 on survival, behavioral tests, and
genetic markers
This trial was conducted at the
animal facility of the University of Zaragoza using the colony of
SOD1G93A mice that has been bred for years by Dr.
Osta’s group. MP-010 was given to 21 SOD1G93A mice
(n=8/13 females/males) in the drinking water at a concentration of 1 mM,
treating the mice at a dose of 61 mg/kg. Simultaneously, another group
of 20 SOD1G93A mice (n=9/11 females/males) was treated
with vehicle as the control group. The treatment started at 8 weeks of
age and was weekly renewed until each mouse reached its respective
humane endpoint. Throughout the treatment, all mice underwent weekly
weight measurements and motor behavioral tests, including rotarod and
hanging-wire tests. All behavioral experiments in the three trials were
conducted under blind conditions regarding the treatment.
Trial 3: Effect of MP-001 on survival, behavioral tests and
genetic markers
This trial was conducted at the animal facility of the University of
Zaragoza using the colony of SOD1G93A mice that has
been bred for years by Dr. Osta’s group. This experimental study
included one group of 19 SOD1G93A mice (n=9/10
females/males) to which compound MP-001 was administered in the drinking
water at a concentration of 0.85 mM, resulting in 61 mg/kg dose, and
another similar group of 17 SOD1G93A mice (n=9/8
females/males) treated with vehicle as control. All mice underwent
weekly weight measurements and behavioral tests, including rotarod and
hanging-wire tests, and the survival time was monitored.