Statistical methods
Data are shown as mean ± SEM. Electrophysiological and functional measurements were analyzed with Two-way ANOVA, applying Bonferroni post-hoc test for multiple comparisons. Histological data were analyzed using One-way ANOVA with Bonferroni multiple comparisons test. The survival time of mice was evaluated using Kaplan-Meier analysis and Log-Rank test. Comparisons of means of gene and protein expression were analyzed by unrelated t-test. The computer-assisted analysis of the bands obtained in western blot was performed with AlphaEase FC software (Bonsai Technologies Group, S.A.). Statistical analysis was performed using SPSS (version 20, IBM, Armonk, NY, USA), and graphs were made using GraphPad Prism Software (version 5, La Jolla, CA, USA) and Microsoft Excel. Statistical significance was set at p<0.05.