Figure 3. Preservation of NMJ Innervation and Spinal MNs in
SOD1G93A Mice upon MP-010 Treatment.
A) Plot illustrating the percentage of neuromuscular junctions
(NMJ) innervated (overlap of signals) in the different mouse groups.
B) Representative confocal images of NMJ in tibialis anterior
(TA) muscles of mice at 16 weeks of age, presented as maximum
projections generated from 1.3 μm z projections. Immunolabeling against
α-bungarotoxin (nicotine receptor, in green), neurofilament 200 +
synaptophysin (synapse, in red) was performed to assess NMJ innervation.
Scale bar: 100 µm.
C) Representative images of lumbar spinal sections depicting
MNs stained with cresyl violet from mice at 16 weeks of age. Scale bar:
100 μm.
D) Plot showing the number of surviving spinal MNs (mean number
of MNs per section ± SEM) in L4-L5 segments at 16 weeks of age in the
different mouse groups.
Mean ± SEM are represented in the plots. Statistical analysis employed
one-way ANOVA with Bonferroni multiple comparisons test. *p <
0.05 compared to the SOD1G93A vehicle group.