Fibrosis and lipid metabolism in c-Jun~Fra-2hep livers.  A. Masson Trichrome staining of c-Jun~Fra-2hep and control liver sections at 2 months. Bar = 100 mm, arrows point to fibrotic areas. B. qRT-PCR quantification of fibrosis-associated genes in c-Jun~Fra-2hep livers compared to controls at 2 months, p<0.05 for each mRNA. C. Overrepresentation analysis (ORA, WebGestalt) of upregulated genes c-Jun~Fra-2hep livers at 2 months (RNAseq, n= 6 controls and 3 mutants) using Pathways/Reactome (enrichment >2.6) and Gene Ontology/Biological processes (GO: BP, enrichment >1.96) terms. The dotted line shows the adjusted FDR cut-off of <0.05. D. Immunoblot for total and phosphorylated Smad2 in liver extracts from c-Jun~Fra-2hep mice (non-tumoral and tumors) and controls. E. Pparg IHC and Oil Red O staining in c-Jun~Fra-2hep (NT) and control livers at 9 months. Bar = 100mm. F. qRT-PCR quantification of pparg2, encoding Pparg in c-Jun~Fra-2hep tumors and non-tumoral (NT) liver areas compared to controls. G. Liver triglycerides (TG) content in c-Jun~Fra-2hep mice compared to controls at 9 months. Vinculin is used to control immunoblot loading. Bars = means ±SEM, n≥3. * p<0.05, ** p<0.01, *** p<0.001 (t-test to controls).