Comparison of Overall Accuracy and Cohen’s kappa coefficient
based on rRT-PCR Ct values for Operators’ ICT Kits
The various rRT-PCR Ct value categories were compared with the overall
accuracy and Cohen’s kappa coefficient to ascertain if the Ct values
affected any of these diagnostic performance indicators. The results
show that the DG Rapid and SD Rapid kits had higher overall accuracy and
Cohen’s kappa coefficient than the SS Rapid kit across all Ct value
categories. The DG Rapid kit had the highest overall accuracy and
Cohen’s kappa coefficient for Ct values of 19.00 - 29.99 and 30.00 -
34.99, while the SD Rapid kit had the second highest overall accuracy
and Cohen’s kappa coefficient for the same ranges. The SS Rapid kit had
the lowest overall accuracy and Cohen’s kappa coefficient across all Ct
value categories. However, for Ct values >35.00, all three
kits recorded the lowest overall accuracy and Cohen’s kappa coefficient.