Comparison of Overall Accuracy and Cohen’s kappa coefficient based on rRT-PCR Ct values for Operators’ ICT Kits
The various rRT-PCR Ct value categories were compared with the overall accuracy and Cohen’s kappa coefficient to ascertain if the Ct values affected any of these diagnostic performance indicators. The results show that the DG Rapid and SD Rapid kits had higher overall accuracy and Cohen’s kappa coefficient than the SS Rapid kit across all Ct value categories. The DG Rapid kit had the highest overall accuracy and Cohen’s kappa coefficient for Ct values of 19.00 - 29.99 and 30.00 - 34.99, while the SD Rapid kit had the second highest overall accuracy and Cohen’s kappa coefficient for the same ranges. The SS Rapid kit had the lowest overall accuracy and Cohen’s kappa coefficient across all Ct value categories. However, for Ct values >35.00, all three kits recorded the lowest overall accuracy and Cohen’s kappa coefficient.