cDC cytokine secretion
Splenic cDC populations were isolated and cell sorted to purity. 1 x 105 cells were incubated in the presence of 0.5 µM CpG, 50 ng/mL interferon gamma (Peptrotech) and 20 ng/mL granulocyte-macrophage colony-stimulating factor (GM-CSF, Peprotech). Supernatants were collected 18 hours later and stored at -20°C. Cytokine secretion was determined using the BD Cytometric Bead Array Mouse Inflammation kit according to the manufacturer’s instructions (BD Biosciences).