mTHPC (meta-tetrakis 3-hydroxyphenylchlorin) is a potent photosensitizer but difficult to deliver: liposomes are limited by a rapid release of the drug or a degradation of the particle. Using extracellular vesicles (eVs) from mesenchymal stem cells in a HT29 spheroid colorectal model (3 zones : necrotic, quiescent, proliferative)\cite{Yakavets2017}. They show deeper diffusion (>100µm) of the loaded eVs (followed by fluorescence)and +50% of phytotoxicity by PDT \cite{Silva2013} . Different sources for building eVs : MSCs, endothelial, platelet, macrophage cells.

Anne-Isabelle Henry - Northwestern university US - Nanoplatforms for label-free biomarkers sensing with SERS

Gold core, organic lining, silica shell for application in Surface-enhanced Raman spectroscopy. With no capture agents the idea is to bind biological compound by entrapment between particles. With 4 amino 3 fluorobiphenylbisboronic acid as a capture agent, it can complex glucose at physiological pH\cite{Henry2016,Henry2017}.

Teresa Pellegrino - Istutito Italianio di tecnologia IT - Inorganic nanocrystals and their assemblies to tackle cancer and neuron diseases

Inorganic particles, Quantum dots(QDs) and Quantum rods (QRs): QDs -23mV, QRs at 80nm -32mV negative charge works, neutral don't = minimum charge for loading in neurons (not glia ) is -20mV, no material specific nor shape ? When they block ion channels with TTX drug on neurons, QRs doesn't interact anymore.

For tuning size they use squalane to stabilize temperature, dibenzylether to homogenize as formaldehyde is volatile. Smaller size are less dependent on viscosity for hysteresis.

Laurent Cognet - Institut d'optique d'Aquitaine, Bordeaux - Nanoscale Imaging of live brain tissue

Chloe Grazon - Boston university US - A nanoparticle sensor for hormones based on a native microbial sensing

Work with Mark Grinstaff team and LCPO Bordeaux. The goal is to design new sensor for progesterone as actual one are Antibody based, so expensive. Quantum dots grafted with hormone sensitive transcription factor (DNA) and DNA labeled with a fluorophore. In resting state FRET is active between labeledDNA and QDs, but with very low amount of progesterone, DNA and transcription factor separates and FRET goes down, only QDs fluorescence is measured.

Xavier Le Guevel - Institute for advanced biosciences Grenoble - Tailoring gold nanocluster surface : from cell interaction towards in vivo studies

Au Nps <10nm coated with 10kDa GSH, analyzed by SWIR imaging. Project with Cyril Richard on ZGO nanoparticles : ZnGa₂O₄Cr³⁺ with PEG, study degradation in ALF medium (artificial lysosomal fluid) .

Thomas Lecuyer - Faculty of pharmacy Paris - Persistent luminescence nanoparticles for in vivo imaging : a better understanding of their fate

Sponsor presentations

SESSION 3 : Bioinspired nanomedecine

Jan Van Hest - Eindhovern university Netherlands - Designer compartments for nanomedecine

Elastin like polypeptide (ELP) contains Val-Pro-Gly-X-Gly pattern with X a residue determining hydrophilic or hydrophobic nature of ELP. Temperature transition affect aggregation. Amphiphilic ELP particle + nanobody against EGFR or cdc50A+ photosensitizer. PDT in coculture possible.

PS-PIAT nanoreactors: enzyme shielded and trapped inside structure, but substrates and products can go in and out = this nano-stomach can self-select features regarding differents cells.

Vladimir Baulin - University of Rovira I Virgili Spain - Active vs passive translocation of nanoparticles through lipid bilayers

Evangelos Georgilis - LCPO Pessac France - Diblock Elastin like polypeptide for the development of a thermo responsive self assembled drug delivery system

Using diblock elastin like polypeptide (ELP) VPGXG with X as isoleucine for hydrophobic block and methionine for hydrophilic block. Oxidizing the thioether group of the methionine to control the hydrohilicity of the the bifunctional block\cite{Meyer1999}.

Marie Morille - Institute Charles Gerhardt Montpellier - Post production modifications of murine mesenchymal stem cells derived extracellular vesicles and evaluation of their cellular interaction compared to synthetic liposomes

Wim Hennik - Utrecht university Netherlands - Nanogels for the intracellular delivery of proteins for cancer treatment

cationic nanogel with dextran, with ovalbumin grafted on it to deliver antigen to dendritic cells. Once inside the cells, GSH cleave bond and release antigen to boost class I MHC presentation. Protein loading with HEPES 20mM pH 7.4. Test release in PBS Nacl, was too fast, in burst, so they use the thiol linker to be conditioned by glutathion concentration. Mice model is positive. This strategy can be use for presenting tumor antigen or deliver terapeutic antigens. They use folate for targeting tumor cells. For loading of cationic protein like ribonuclease A, they use anionic hydrogel coated dextran and reverse charge particle surface with PEI.

SESSION 4

Jinjun Shi - Harvard university - Nanomedicine for cancer and ischemic brain damage

Albumin grab lipidPEG and allow sustained release; They study prohibitin in non small cell lung cancer and siRNA PHB1 vectorized in nanoparticles is effective; Restore BRAF mutation in thyroid cancer.

Jeanne Chain-Leblond- University of Montreal, Canada- Cationic switchable lipid nanoparticles for a combination treatment of retinoblastoma

pH cationic switchable lipids (CSL) nanoparticles, to carry mIR181a and melphalan for a total size at 160nm. Uptake and better antiproliferative effects and reduce therapeutic concentration of intravitreal injection \cite{Viricel2017}.

Veronique Gigoux - Receptology and targeted therapy of cancers, Toulouse - Targeted magnetic intralysosomal hyperthermia produces lysosomal reactive oxygen species and causes caspase 1 dependant cell death

Magnetic intra-lysosomal hyperthermia (MILH) with Fe₃O₄ 10nm, targeting CCK2R. In high frequency AMF, no temperature rise in cell medium. Kill tumor with AMF application through lysosomal death. Wanted effect of lysosomal fenton reaction (positive control with FeCl₃₎ causing lipid peroxidation cathepsin B release and caspase 1 activation but not caspase 3.

Romain Grall - Laboratory of experimental cancerology, CEA Fontenay - Metallic nanoparticles to impair cancer radioresistance : impact for tumor heterogeneity and study of long term effects on biological conclusions

Radiation resistance marker in breast cancer (MDAMB231, T47D): CD44/24 Gold and platinum nanoparticles with PEG for radiosensitizing.

Hamideh Salehi - laboratory of bioengineering, Montpellier - Application of dental pulp stem cells as anticancer drugs transporters for chemotherapy

Dental pulp is the less invasive source for MSCs and can be loaded with palclitaxel exerting no viability loss. Time dependant release is measured in culture medium of cancer cells. They need to follow the fate of the drug without labelling : use of Confocal raman spectroscopy\cite{Salehi2013,Salehi_2013}.

Caroline Bissardon - Synchroton radiation and medical research, Grenoble - Role of selenium nanoparticles to dampen the metastatic potential of agressive cancers.

Elise Lepeltier - MINT lab, Angers - Lipid nanocapsules entered preferentially into human glioblastoma cells compared to normal human astrocytes after surface fonctionnalization with NFL petide

Working on glioblastoma for delivering ferrocifen, using lipidnanocapsules. Prepared by emulsion phase inversion . They test different uptake depending on the targeting peptide NFL present (grafted ? ) at surface\cite{Lainé2014}.

Guy Kantor - Institut Bergonié , Bordeaux - Radio enhancement with nanoparticles. First human clinical trials

Vladimir Mulens - MSC , Paris - Fractal rearrangement induced by 5 fluorouracil to trigger UV-visible light absorbent gold nanoparticle plasmon coupling for the therapeutic synergy of mild NIR photo and chemotherapy in colon carcinoma and carcinomatosis treatment

Flavia Fontana- Drug research program Helsinki university, Finland - Biohybrid nanovaccines in murine melanoma: Efficacy and synergistic combo therapy with immune checkpoint inhibitor

Porous silicon nanoparticles coated with acetalated dextran coated with antigen from tumor membrane; mice treated showed remission. Combo with an anti-CTLA-4 checkpoint inhibitor together with the biohybrid nanovaccine = increased cytotoxic lymphocytes within tumor environment\cite{Fontana2017}.

Koen Raemdonck - Laboratory of general biochemistry and physical pharmacy Ghent university, belgium - Repurposing low molecular weight drug as adjuvants to induce lysosomal

siRNA escape in nanogel transfected cells

Lysosome is seen as an end point but in these case they different fda-approved drugs repurpose for permeabilizing lysosomes and release siNGs, cationic amphiphilic drugs treatment after siNGs treatmen could trigger multiple rounds if siRNA release. The use for instance SMA ( Sphingomyeloid acid ). Staining with LipidTOX for lipids inside cells.

Dominika Hobernik - department of dermatology Meinz university, Germany - Efficient gene delivery and simultaneous immune cell activation by LAH4-L1 based nanoparticles ? new perspectives for DNA vaccines ?

Kathia Lemdani - Paris V university with N. Mignet and D.Scherman - GMCSF loaded hydrogel improve antitumor immune response in colorectal cancer

Anna Lechanteur - GIGA Cancer Liège university , Belgium - Promoting vaginal distribution of two active siRNA complexed in liposomes for cervical cancer treatment

Liposomes + PEG stay stuck in mucosa, possibly due to steric hindrance from PEG, limiting siRNA delivery in mucosal cancer. study different kind of mixtures of PEG to bypasse this issue. Best knockdown with 80% DSPE-PEG₂₀₀₀ 20%ceramide-PEG₂₀₀₀

Roberta Guagliardo - Laboratory of general biochemistry and physical pharmacy Ghent university, belgium - Pulmonary surfactant coated nanogels for siRNA delivery : role of surfactant protein B

core based on biodegradable polysaccharide nanogel loaded with siRNA and a shell from natural-derived pulmonary surfactant 90%lipids 10%lipid. This shell stabilizes the polymeric core and specifically, surfactant protein B (SPB), is involved in increasing siRNA transmembrane delivery.

Marie Millard - institute of cancerology of Lorraine - Influence of mTHPC nanovectorization by extracellular vesicles on the photodynamic therapy efficacy.