Fig. 4: Temporally controlled (ubiquitous) expression of tßh in tßhnM18 mutant background.
A. Temperature shift 3h before the test. Flies with a rescue construct show an intermediate PER level, significantly different from both the mutant and the control flies. B. Temperature shifts during the starvation period, but not immediately before the test. Flies with a rescue construct behave similarly as mutant flies. Total number of proboscis extension responses is represented in boxplots (see Fig.1). Numbers indicate sample size, asterisks denote significant difference between groups (paired Wilcoxon rank sum test, p < 0.05).