Flies were raised on
cornmeal-yeast-glucose-agar medium under a 12/12 h light/dark
cycle (lights on at 06:00 hrs) at 25°C.
Newly hatched to one day old flies were collected and transferred
into a vial containing Kimwipe paper soaked with
100 mM glucose for one to two days as previously described Zhang 2010. Starved flies were kept in a vial containing
Kimwipe paper soaked with Evian® water for 20 h before testing.