tßhnM18 (
Monastirioti 1996;
FBal0061578),
oamb (
Han 1996; OctαR,
oamb286 FBal0152344,
oamb584 FBal0152335),
honoka
(Kutsukake 2000; Oct-TyrR, FBal0104701),
hsp-tßh (
Schwaerzel 2003; FBal0152162) and w
+;;UAS-
tßh (
Monastirioti 2003; FBti0038601) and their control lines were obtained from Henrike
Scholz, Cologne; Hiromu Tanimoto, Martinsried; Andreas Thum,
Konstanz; and Amita Seghal, Chevy Chase.
TyrRf05682 (
CG7431f05682, FBal0184987),
TyrRII∆29 (CG16766, FBgn0038541) and
TyrRII-TyrR∆124 were kindly provided by Edward Blumenthal,
Milwaukee
Zhang 2017. Receptor mutants
(and the respective control lines we obtained from the different labs) were outcrossed for at least six
generations into a CS background.
Flies were kept on standard cornmeal/molasses-food in a 12/12 h
light/dark cycle (light on at 8:00 hrs) at 60% relative humidity
and 25°C except for hsp-tßh, which were raised at 18°C
without humidity control and except for flies used in
electrophysiological experiments (see Electrophysiological
recordings).