Newly hatched to one day old flies were collected and transferred to
fresh food vials. The following day, flies were briefly
CO2-anesthetized and sorted by and genotype. At
17:00 hrs, around 20 female flies were transferred into a
starvation vial (see Starvation procedure). Dead flies were counted
every 3 h and not removed. Daily counting sessions were repeated
from 9:00 to 18:00 hrs, until all flies were found dead.