For the first sugar response rescue attempt (Fig. 4A), flies were raised and starved at 18°C and put into an incubator without humidity control and heated up to 37°C for 30 to 45 min. After the heat shock, flies were kept in a 25°C incubator with humidity control for 3 h until testing. For the second rescue attempt (Fig. 4B), the first heat shock was given with beginning of starvation every 23 h for 45 min until one day before testing. Temperature between heat shocks was 18°C.