In order to elucidate the temporal requirement of tßh activity during starvation or during proboscis extension, we induced ubiquitous, but temporally controlled, tßh expression in the mutant background using the heat-inducible hsp-tßh construct. To prevent tßh expression, flies were kept at 18 degrees, and the starvation time had to be increased to get wild type flies to respond to the sugar stimulation.  Driving expression 3 h before testing the sugar response partially rescued the mutant phenotype (Fig. 4A, Wilcoxon rank sum test with Bonferroni correction, p<0.05). In contrast, heat shocks throughout the starvation period did not rescue the sugar response phenotype (Fig. 4B), suggesting an acute role of OA during the sugar response test, independent of any OA/TA role in starvation resistance.