Hemolymph carbohydrate content was calculated based on a calibration value showing the absorbance of a standard glucose/trehalose solution that was treated identically to hemolymph (one value was taken each day). In the 2 days with two measures per group, values were paired following time of measurement. Statistical analysis was performed using a paired Wilcoxon rank sum test on the index of change in sugar content with starvation: (SG_starved - SG_fed)/(SG_starved + SG_fed)