Trehalose and glucose content in the hemolymph was measured according to the protocols provided by the manufacturer (Sigma Aldrich, Seelze, Germany). 10 µl of the hemolymph-PBS mixture (or calibration solution) were added to 30 μl citric acid buffer (135 mM, pH 5.7 at 37°C) and 10 μl of a trehalase enzyme solution (Sigma Aldrich, 3% in citric acid buffer). After incubation overnight at 37°C, 50 μl of Tris buffer were added. 80 µl of the resulting solution were added to 156.8 μl Glucose oxidase and 3.2 μl o-Dianisidine (Glucose Assay Kit, Sigma Aldrich) and incubated for 30 min at 37°C. Finally, 160 μl of 33% sulfuric acid were added. Absorbance at 540nm was measured for the resulting solution using a nanoDrop® (nanoDrop Technologies, Wilmington, USA) spectrophotometer. Five samples were measured per solution.