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Consolidated bioprocessing of hemicellulose enriched lignocellulose to succinic acid through a microbial co-cultivation system
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  • Jiasheng Lu,
  • Yang Lv,
  • Yujiang Jiang,
  • Min Wu,
  • Bin Xu,
  • Wenming Zhang,
  • Jie Zhou,
  • Weiliang Dong,
  • Fengxue Xin,
  • Min Jiang
Jiasheng Lu
Nanjing Tech University
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Yang Lv
Nanjing Tech University
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Yujiang Jiang
Nanjing University of Technology
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Min Wu
Nanjing Tech University
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Bin Xu
Nanjing Tech University
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Wenming Zhang
Nanjing University of Technology
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Jie Zhou
Nanjing Tech University
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Weiliang Dong
Nanjing University of Technology
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Fengxue Xin
Nanjing University of Technology
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Min Jiang
Nanjing University of Technology
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Abstract

Consolidated bioprocessing (CBP) has been widely adopted as a cost-effective strategy for the bioconversion of lignocellulosic biomass into bio-chemicals. Microbial consortium can complete the complex CBP processes through the cooperation of different microorganisms. In this study, a synthetic microbial consortium was designed, which is composed of a hemicellulase-producing bacterium Thermoanaerobacterium thermosaccharolyticum and succinic acid production specialist Actinobacillus succinogenes 130Z. The simultaneous conversion of xylose hydrolyzed by T. thermosaccharolyticum could maintain a high hydrolyzing rate, which would facilitate succinic acid production by A. succinogenes 130Z. After process optimization, 32.50 g/L of succinic acid with yield of 0.41 g/g was obtained from 80 g/L xylan through CBP, representing the highest succinic acid production directly from hemicellulose materials. In addition, 12.51 g/L of succinic acid was directly produced from 80 g/L of corn cob. The above results demonstrated that this CBP based microbial co-cultivation system had great potential to convert lignocellulosic biomass into various bio-chemicals.