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Comparison Study of Stem Cell-Derived Extracellular Vesicles for Bone Tissue Engineering
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  • Elham Pishavar,
  • Joshua Copus,
  • Anthony Atala ,
  • Sang Jin Lee
Elham Pishavar
Wake Forest School of Medicine
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Joshua Copus
Wake Forest School of Medicine
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Anthony Atala
Wake Forest School of Medicine
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Sang Jin Lee
Wake Forest School of Medicine
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Abstract

Stem cell-derived extracellular vesicles (EVs) have shown great promise in the field of regenerative medicine and tissue engineering. Recently, human bone marrow-derived mesenchymal stem cell (BMSC)-derived EVs have been considered for bone tissue engineering applications. In this study, we evaluated the osteogenic capability of placental stem cell (PSC)-derived EVs and compared them to the well-characterized BMSC-derived EVs. EVs were extracted from three designated time points (0, 7, and 21 days) after osteogenic differentiation. The results showed that the PSC-derived EVs had much higher protein and lipid concentrations than EVs derived from BMSCs. The extracted EVs were characterized by observing their morphology and size distribution before utilizing next-generation sequencing to determine their miRNA profiles. It was identified that 306 miRNAs within the EVs, of which 64 were significantly expressed in PSC-derived EVs that related to osteogenic differentiation. In vitro osteogenic differentiation study indicated that the late-stage (21-day extracted) derived EVs higher osteogenic enhancing capability when compared to the early-stage derived EVs. We demonstrated that EVs derived from PSCs could be a new source of EVs for bone tissue engineering applications.

Peer review status:POSTED

02 Jul 2020Submitted to Biotechnology Journal
06 Jul 2020Assigned to Editor
06 Jul 2020Submission Checks Completed