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Molecular characterization of MHC class I genes in Four species of Turdidae family to assess genetic diversity and selection.
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  • Muhammad Usman Ghani,
  • Shakeel Hussain,
  • An Buyang,
  • Xu Yanchun,
  • Li Bo
Muhammad Usman Ghani
Northeast Forestry University
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Shakeel Hussain
Northeast Forestry University
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An Buyang
Northeast Forestry University
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Xu Yanchun
Northeast Forestry University
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Abstract

In vertebrate animals, the molecules encoded by major histocompatibility complex (MHC) genes play an essential role in the adaptive immunity. MHC class I deal with intracellular pathogens (Virus) in birds. MHC class I diversity depends on the consequence of local and global environment selective pressure and gene flow. Here, we evaluated the MHC class I gene in four species of Turdidae family from a broad geographical area of northeast China. We isolated 77 MHC class I sequence, including 47 putatively functional sequences and 30 pseudo sequences from 80 individuals. Method based on analysis of cloned amplicons (n=25) for each species, we found two and seven MHC I sequence per individual indicates more than one MHC I loci identified in all sampled species. Results revealed an overall elevated genetic diversity at MHC class I, evidence of different selection patterns among the domains of PBR and non-PBR. Alleles found to be divergent with overall polymorphic sites per species ranging between 58 to 70 (out of 291 sites). Moreover, trans-species alleles were evident due to convergent evolution or recent speciation for the genus. Phylogenetic relationships among MHC I show an intermingling of alleles clustering among Turdidae family rather than between other passerines. Pronounced MHC I gene diversity is essential for existence of species. Our study signifies a valuable tool for the characterization of evolutionary relevant difference across a population of birds with high conservational concerns.

Peer review status:Published

10 Apr 2021Published in BioMed Research International volume 2021 on pages 1-14. 10.1155/2021/5585687