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Molecular detection of Toxoplasma gondii in placentas of women who received therapy during gestation in a toxoplasmosis outbreak
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  • Aline Ludwig,
  • Fagner Fernandes,
  • Renata Rojas Guerra,
  • Camila Minuzzi,
  • Patricia Braunig,
  • Alisson Rodrigues Döhler,
  • Luciane Ramos,
  • Liliane Souto Pacheco,
  • Luis Antonio Sangioni,
  • Fernanda Silveira Flores Vogel
Aline Ludwig
UFSM
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Fagner Fernandes
Universidade Federal de Santa Maria
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Renata Rojas Guerra
UFSM
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Camila Minuzzi
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Patricia Braunig
Universidade Federal de Santa Maria
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Alisson Rodrigues Döhler
UFSM
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Luciane Ramos
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Liliane Souto Pacheco
UFSM
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Luis Antonio Sangioni
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Fernanda Silveira Flores Vogel
UFSM
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Abstract

Toxoplasmosis is a disease caused by T. gondii, a protozoa which affects humans and animals and is widely distributed worldwide. In humans, there is great concern due to the serious consequences that can occur in the infection of pregnant women and the newborn. The early diagnosis of gestational toxoplasmosis is important for treatment to be carried out in order to prevent vertical transmission or reduce damage. The diagnosis can be made through the detection of antibodies in pregnant women or neonates and PCR of amniotic fluid. Previous studies have also reported PCR of the placenta as a good diagnostic test. Our study evaluated the detection of T. gondii DNA in placenta samples from parturients seen at the University Hospital of Santa Maria, Southern Brazil and treated during the pregnancy. We performed PCR in forty samples and five were positive, representing 12.5%. When correlating the treatment time and the detection of DNA in the placentas, no significant result was found. The prevalence of positive samples was lower than in other studies in the literature. The data reaffirm the importance of carrying out the analysis of the placenta. Key words: Placenta, PCR, toxoplasmosis.