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Immune Responses in Mice Vaccinated with Virus-Like Particles of Western Equine Encephalitis Virus from an Insect Cell-Based Expression System
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  • Jinzhu Ma,
  • Hualei Wang,
  • Xuexing Zheng,
  • Hongxia Wu Wu,
  • Songtao Yang,
  • XIanzhu Xia
Jinzhu Ma
Academy of Military Medical Science of Chinese People's Liberation Army
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Hualei Wang
Academy of Military Medical Science of Chinese People's Liberation Army
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Xuexing Zheng
Academy of Military Medical Science of Chinese People's Liberation Army
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Hongxia Wu Wu
Academy of Military Medical Science of PLA
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Songtao Yang
Academy of Military Medical Science of Chinese People's Liberation Army
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XIanzhu Xia
Academy of Military Medical Science of Chinese People's Liberation Army
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Abstract

Western equine encephalitis virus (WEEV) can cause lethal encephalitis in humans and equines and represents a serious public health threat in many countries. Therefore, development of efficient vaccines against WEEV remains an important challenge in the field of disease control. This study described for the first time successful production of WEEV virus-like particles (VLPs) in insect cells using recombinant baculoviruses. This well-established expression system is very suitable for production of WEEV VLPs. The immune experiment herein in mice showed that the VLPs formulated with 206-adjuvant were responsible for the stronger-VLP-specific cellular immune response, and were able to induce the secretion of IL-2, IL-4, IFN-γ and production of high titer antibodies that can effectively neutralize the WEEV pseudoviruses. The WEEV VLPs from insect cells could provide a new, safe, non-replicating and effective vaccine candidate against WEEV infections.

Peer review status:UNDER REVIEW

06 Jan 2021Submitted to Biotechnology Journal
06 Jan 2021Submission Checks Completed
06 Jan 2021Assigned to Editor
08 Jan 2021Reviewer(s) Assigned
02 Feb 2021Editorial Decision: Revise Major
05 Apr 20211st Revision Received
09 Apr 2021Submission Checks Completed
09 Apr 2021Assigned to Editor
09 Apr 2021Reviewer(s) Assigned
28 Apr 2021Editorial Decision: Revise Major
12 May 20212nd Revision Received
12 May 2021Submission Checks Completed
12 May 2021Assigned to Editor
12 May 2021Reviewer(s) Assigned
27 May 2021Editorial Decision: Revise Minor
28 May 20213rd Revision Received
29 May 2021Assigned to Editor
29 May 2021Submission Checks Completed
29 May 2021Reviewer(s) Assigned