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EVALUATION OF INDUCED SPUTUM CYTOLOGY IN ASTHMATIC CHILDREN
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  • Debora Carla Chong-Silva,
  • Adriana Nascimento,
  • Roberta Cunha,
  • Elessandra Bitencourt,
  • Leticia Botelho,
  • Ana Beatriz Dantas,
  • Cristine Rosario,
  • Carlos Riedi,
  • Herberto Chong-Neto,
  • Denise Eli,
  • Nelson Rosario Filho
Debora Carla Chong-Silva
Federal University of Parana
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Adriana Nascimento
Federal University of Parana
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Roberta Cunha
Federal University of Parana
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Elessandra Bitencourt
Federal University of Parana
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Leticia Botelho
Federal University of Parana
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Ana Beatriz Dantas
Federal University of Parana
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Cristine Rosario
Federal University of Parana
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Carlos Riedi
Federal University Parana
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Herberto Chong-Neto
Federal University of Parana
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Denise Eli
Federal University of Parana
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Nelson Rosario Filho
Universidade Federal do Paraná
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Abstract

Objective: To evaluate the viability of sputum cytology in asthmatic children, recognizing inflammatory patterns and correlating them with clinical, epidemiological and functional variables of the disease. Methods: This was a cross-sectional and observational study of children with asthma who underwent sputum induction through increasing concentrations of nebulized hypertonic saline solution from 3% to 7%. The samples were processed according to the technique developed by Pizzichini et al. and the cytological pattern classified as pauci-granulocytic, neutrophilic, eosinophilic and mixed. Samples with cell viability> 50% were considered adequate. Asthma control was assessed using the asthma control test (ACT). Results: Seventy-nine children performed sputum induction. Thirty-three samples were excluded because they were not viable for analysis, resulting in 46 samples. The children’s average age was 9.4 (± 3) years. There was a predominance of eosinophilic (25/46, 54.3%), followed by mixed (13/46, 28.3%), pauci-granulocytic (7/46, 15.2%) and neutrophilic (1/46, 2.2%) pattern. Sixty-three percent of the children had severe asthma and 84.7% were treated with inhaled corticosteroids. The ACT showed that 25 (56.8%) patients had the disease under control. Forty-five children (97.8%) underwent pulmonary function tests (spirometry) and in 13 cases (28.9%) an obstructive ventilatory defect was found. Conclusions: The eosinophilic profile was predominant in the assessed asthmatic children. Non-eosinophilic phenotypes were found, but less frequently. There was no difference between the clinical variables and the sputum profile in this study group. Sputum induction in children with asthma is feasible and safe and can contribute to a specific and personalized approach to the disease.