Abstract
Analysis of transcription factors and chromatin modifications at the
genome-wide level provides insights into gene regulatory processes, such
as transcription, cell differentiation and cellular response. Chromatin
immunoprecipitation is the most popular and powerful approach for
mapping chromatin, and other enzyme-tethering techniques have recently
become available for living cells. Among these, CUT&Tag is a relatively
novel chromatin profiling method that has rapidly gained popularity in
the field of epigenetics since 2019. It has also been widely adapted to
map chromatin modifications and TFs in different species, illustrating
the association of these chromatin epitopes with various physiological
and pathological processes. Scalable single-cell CUT&Tag can be
combined with distinct platforms to distinguish cellular identity,
epigenetic features and even spatial chromatin profiling. In addition,
CUT&Tag has been developed as a strategy for joint profiling of the
epigenome, transcriptome or proteome on the same sample. In this review,
we will mainly consolidate the applications of CUT&Tag and its
derivatives on different platforms, give a detailed explanation of the
pros and cons of this technique as well as the potential development
trends and applications in the future.