Antihypertensive and Hepatoprotective activity of optimized eupalitin
3-O- β-D-galactopyranoside from Boerhavia diffusa Linn.
Abstract
Background and Purpose: Boerhavia diffusa is an herbaceous member of the
Nyctaginaceae family which has been widely used in folk medicine to
treat several illnesses. Experimental Approach: A validated HPTLC method
has been newly developed for the quantification of eupalitin 3-O-
β-D-galactopyranoside in hydroalcoholic extracts of B. diffusa. A
three-level factor Box-Behnken statical design was used for
optimization, extraction time (min), temperature (ºC), and methanol:
water ratio (% v/v) are independent variables while bioactive compounds
as the dependent variable. This study was aimed to investigate the
pretreatment of HepG2 cells with hepatoprotective agents against the
damage induced by galactosamine (GalN) and evaluate in vivo
antihypertensive activity of eupalitin 3-O- β-D-galactopyranoside in
rats by prednisolone inducing hypertension. Key Results: The separation
was achieved on silica gel 60F254 HPTLC plate using: toluene: acetone:
water (5: 15:1) as mobile phase. Densitometric analysis of eupalitin
3-O- β-D-galactopyranoside was carried out in the absorbance mode at 366
nm. A study has shown that optimized eupalitin 3-O-β-D-galactopyranoside
can be used as an antihypertensive drug in rats by prednisolone inducing
hypertension and In-vitro Hepatoprotective Activity of optimized
eupalitin-O- β-D-galactopyranoside was checked in HPG2 cell line
compared with silymarin by ANOVA technique. Conclusion and Implications:
It is concluded that A new method for a method of validation and
quantification of eupalitin-O-β-D-galactopyranoside in Boerhavia diffusa
has been developed, In vivo antihypertensive and in-vitro
hepatoprotective activity of optimized eupalitin O-
β-D-galactopyranoside was done in intoxicated rats and in HepG2 cells
induced by galactosamine (GalN).