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Integrated full-length transcriptome and metabolome analysis reveals the defense response of melon to gummy stem blight
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  • Qiusheng Kong,
  • Haiyan Wang,
  • Xiaoying Wei,
  • Changjuan Mo,
  • Minghua Wei,
  • Yaqiong Li,
  • Yuxin Fan,
  • Xiaojing Gu,
  • Xuejun Zhang,
  • Yongbing Zhang
Qiusheng Kong
Huazhong Agriculture University College of Horticulture and Forestry Sciences

Corresponding Author:[email protected]

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Haiyan Wang
Huazhong Agriculture University College of Horticulture and Forestry Sciences
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Xiaoying Wei
Huazhong Agriculture University College of Horticulture and Forestry Sciences
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Changjuan Mo
Huazhong Agriculture University College of Horticulture and Forestry Sciences
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Minghua Wei
Huazhong Agriculture University College of Horticulture and Forestry Sciences
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Yaqiong Li
Huazhong Agriculture University College of Horticulture and Forestry Sciences
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Yuxin Fan
Huazhong Agriculture University College of Horticulture and Forestry Sciences
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Xiaojing Gu
Huazhong Agriculture University College of Horticulture and Forestry Sciences
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Xuejun Zhang
Xinjiang Academy of Agricultural Sciences
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Yongbing Zhang
Xinjiang Academy of Agricultural Sciences
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Abstract

Gummy stem blight (GSB), a severe and widespread disease causing great losses to cucurbit production, is a major threat to melon production. However, the melon-GSB interaction remains largely unknown, which significantly impedes the genetic improvement of melon for GSB resistance. Here, full-length transcriptome and widely targeted metabolome were used to reveal the early defense responses of resistant (PI511890) and susceptible (Payzawat) melon to GSB. Differentially expressed genes were specifically enriched in the secondary metabolite biosynthesis and MAPK signaling pathway in PI511890, while in carbohydrate metabolism and amino acid metabolism in Payzawat. More than 1000 novel genes were identified in PI511890, which were enriched in the MAPK signaling pathway. There were 11,793 alternative splicing events identified and involved the defense response to GSB. A total of 910 metabolites were identified, with flavonoids as the dominant metabolites. Integrated full-length transcriptome and metabolome analysis showed that eriodictyol and oxalic acid may be used as marker metabolites for GSB resistance in melon. Moreover, post-transcription regulation was widely involved in the defense response of melon to GSB. These results improve our understanding of the interaction between melon and GSB and may facilitate the genetic improvement of GSB resistance of melon.
Submitted to Plant, Cell & Environment
30 Jan 20241st Revision Received
31 Jan 2024Submission Checks Completed
31 Jan 2024Assigned to Editor
01 Feb 2024Review(s) Completed, Editorial Evaluation Pending
05 Feb 2024Reviewer(s) Assigned
12 Feb 2024Editorial Decision: Accept