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Multi-scale characterization of cold response reveals immediate and long-term impacts on cell physiology up to seed composition
  • +11
  • N. Langlade,
  • Leconte Jean M.L.,
  • Moroldo Marco,
  • N. Blanchet,
  • Bindea Gabriela,
  • Carrère Sébastien,
  • Catrice Olivier,
  • Comar Alexis,
  • Labadie Marc,
  • Marandel Rémy,
  • N. Pouilly,
  • Tapy Camille,
  • Paris Clémence,
  • Mirleau-Thébaud Virginie
N. Langlade
Universite de Toulouse

Corresponding Author:[email protected]

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Leconte Jean M.L.
Universite de Toulouse
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Moroldo Marco
Universite de Toulouse
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N. Blanchet
Universite de Toulouse
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Bindea Gabriela
INSERM
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Carrère Sébastien
Universite de Toulouse
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Catrice Olivier
Universite de Toulouse
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Comar Alexis
Hiphen
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Labadie Marc
Hiphen
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Marandel Rémy
Universite de Toulouse
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N. Pouilly
Universite de Toulouse
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Tapy Camille
Universite de Toulouse
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Paris Clémence
Syngenta France SAS
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Mirleau-Thébaud Virginie
Syngenta France SAS
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Abstract

Early sowing can help summer crops escape drought and can mitigate the impacts of climate change on them, but it exposes them to cold stress during initial developmental stages, which has both immediate and long-term effects on development and physiology. To understand how early night-chilling stress impacts plant development and the yield, we studied the reference sunflower line XRQ under controlled, semi-controlled and field conditions. We performed high-throughput imaging of the whole plant parts and obtained physiological and transcriptomic data from leaves, hypocotyls and roots. We observed morphological reductions in early stages under field and controlled conditions, with a decrease in root development, an increase in reactive oxygen species content in leaves and changes in lipid composition in hypocotyls. A long-term increase in leaf chlorophyll suggests a stress memory mechanism that was supported by transcriptomic induction of histone coding genes. We highlighted leaf transcriptomes in cold-acclimation genes such as chaperone, heat shock and late embryogenesis abundant proteins. We identified genes in hypocotyls involved in lipid, cutin, suberin and phenylalanine ammonia lyase biosynthesis and ROS scavenging. This comprehensive study describes new phenotyping methods and candidate genes to understand phenotypic plasticity better in response to chilling and study stress memory in sunflower.
19 Feb 2024Submitted to Plant, Cell & Environment
19 Feb 2024Submission Checks Completed
19 Feb 2024Assigned to Editor
20 Feb 2024Review(s) Completed, Editorial Evaluation Pending
20 Feb 2024Reviewer(s) Assigned
11 Mar 2024Editorial Decision: Revise Minor
28 Apr 2024Review(s) Completed, Editorial Evaluation Pending
28 Apr 2024Editorial Decision: Accept