Groundwater-surface water interactions are important in controlling lake water residence time, biogeochemistry, and water availability for downstream communities in tropical volcanic catchments. To better understand these complex seasonal interactions, a multi-tracer approach including water and inorganic carbon stable isotopes (δ2H, δ18O, δ13CDIC), hydrochemistry, and 222Rn was applied in Lake Hule, northern Costa Rica. Seasonal isotope mass balance calculations using lake, stream, precipitation, and groundwater isotope compositions were supplemented with local hydrometeorological information. Evaporation to inflow ratios (E/I) revealed a small variability between the dry (December-April) and wet seasons (May-November), with relatively low evaporation losses, 2.9±1.0 % and 3.2±1.8 %, respectively. Bayesian end-member analysis indicated that annual inputs from groundwater, precipitation, and runoff represented 61.3±8.1%, 24.4±8.4, and 14.3±5.9% of total inflow, respectively. Temporal variations of δ13CDIC also confirmed the key role carbonate buffering plays in this lake and indicated greater CO2 degassing from groundwater sources in the wet season. This first tracer-aided assessment in a volcanic lake maar of northern Costa Rica provides evidence of previously unknown groundwater-surface water interactions and poses a promising tool for estimating seasonal variability of groundwater discharge into natural lakes across the volcanic front of Central America.