The study was aimed to evaluate the antioxidant and antiaging potential of Biofield Energy Healing Treatment (the Trivedi Effect®) on a novel test formulation in in vitro. The test formulation was divided into two parts. One part was denoted as the control, without any Biofield Treatment, while the other part was defined as the Biofield Energy Treated test formulation. MTT assay showed that the test formulation was observed as safe upto 100 µg/mL in both human foreskin fibroblasts-1 (HFF-1) and mouse preadipocytes (3T3-L1) cells. Moreover, the Biofield Treated test formulation showed 35.73% and 122.07% increased cellular protection (cell viability) at 25 and 50 µg/mL, respectively in H 2 O 2 induced oxidative damage as compared to the untreated. Collagen was significantly increased by 16.81% and 30.22% in the Biofield Treated test formulation at 75 and 100 µL, respectively as compared to the untreated. Further, cellular senescence was significantly reduced by 66.35% and 22.5% in the Biofield Treated test formulation at 50 and 100 µg/mL, respectively compared to the untreated. Overall, data suggested that the Biofield Energy Treated test formulation can significantly improve antioxidative properties and would be use against antiaging, autoimmune and inflammatory diseases, stress management and prevention by improving overall health.