Pyridoxine hydrochloride is an important cofactor for the regulation of various enzymatic activities and used in the treatment of vitamin B6 deficiency and associated diseases. This study was aimed to analyse the impact of the Trivedi Effect®-Energy of Consciousness Treatment on the physicochemical, spectral, and thermal properties of pyridoxine hydrochloride. The pyridoxine sample was equally divided into control/ untreated and the Biofield Energy Treated pyridoxine. The treated pyridoxine sample received the Biofield Energy Treatment (the Trivedi Effect®) remotely for ~3 minutes by Mr. Mahendra Kumar Trivedi, who was located in the USA, while the test samples were located in the research laboratory in India. The treated sample was designated as the Biofield Energy Treated sample. The analysis revealed that the particle size values at d10, d50, d90, and D(4,3) in the Biofield Energy Treated sample were significantly altered by -5.97%, -0.70%, 20.26%, and 14.15%, respectively compared to the control sample. Subsequently, the specific surface area of the treated sample was significantly increased by 3.22% than the control sample. Besides, the PXRD analysis showed that the relative intensities as well as the crystallite sizes of various characteristic peaks in the treated sample were significantly altered from -7.50% to -41.32% and -38.48% to 9.10%, respectively compared with the control sample. Also, the treated sample showed a significant reduction of 6.23% in the average crystallite size compared with the control sample. The thermal analysis involving the TGA study revealed the significant reduction in the weight loss in 1st and 3rd steps of degradation of the treated sample by 19.05 and 3.03%, respectively along with 1.02% decrease in the total weight loss compared with the control sample. Thus, the thermal stability of the treated sample was observed to be increased compared to the control sample. The DSC analysis showed a slight reduction in the melting point of the treated sample, however, the latent heat of fusion was increased by 15.65% compared with the control sample. Thus, the Trivedi Effect® might help in producing a novel polymorphic form of pyridoxine hydrochloride, which may show improved solubility, dissolution rate, and bioavailability compared with the untreated sample. This Biofield Energy Treated pyridoxine hydrochloride might be beneficial in designing a better pharmaceutical and nutraceutical formulation in terms of better therapeutic responses against vitamin B6 deficiency, with microcytic anemia, autoimmune disorders, Crohn’s disease, ulcerative colitis, glossitis, dermatitis with cheilosis, pyridoxinedependency seizures, electroencephalographic abnormalities, metabolic disorders, celiac disease, pulmonary tuberculosis, hyperhomocysteinemia, dysmenorrhea, etc.