L-cysteine is an essential amino acid that helps in improving the overall health status of humans. The objective of the study was to determine the impact on the physicochemical and thermal properties of L-cysteine after the Trivedi Effect®-Consciousness Energy Healing Treatment using modern analytical techniques. The control sample was kept without any treatment, while the Biofield Treatment was given to the treated sample remotely by a renowned Biofield Energy Healer, Gopal Nayak. The particle size values were significantly reduced by 11.96%(d10), 9.01%(d50), 4.92%(d90), and 7.66% [D (4, 3)], thus, the specific surface area was significantly increased by 14.28% of the treated L-cysteine compared to the control sample. The peak intensities and crystallite sizes were altered ranging from -86.73% to 456.65% and -4.91% to 451.22% respectively, however, the average crystallite size was significantly increased by 87.58% in the treated sample compared to the control sample. The latent heat of decomposition for the 1st and 2nd peak of the treated sample was increased by 12.77% and 5.42% respectively, compared with the control sample. The weight loss was increased by 9.35%; however, the residual weight was significantly reduced by 85.32% in the treated sample compared to the control sample. The maximum thermal degradation temperature of the treated sample was increased by 2.87% compared to the control sample. Hence, the Biofield Energy Treated L-cysteine might show better solubility, dissolution, bioavailability, and be more thermal stability in the pharmaceutical formulations, which would be more efficacious in the treatment of diabetes, cancer, psychosis, and seizures compared to the control sample