loading page

Insulin production from hiPSC-derived pancreatic cells in a novel wicking matrix bioreactor
  • +2
  • Nooshin Amini,
  • Janet Paluh,
  • Yubing Xie,
  • Vinit Saxena,
  • Susan T. SharfsteinOrcid
Nooshin Amini
SUNY Polytechnic Institute
Author Profile
Janet Paluh
SUNY Polytechnic Institute
Author Profile
Yubing Xie
SUNY Polytechnic Institute
Author Profile
Vinit Saxena
Sepragen Corporation
Author Profile
Susan T. Sharfstein
Orcid
SUNY Polytechnic Institute
Author Profile

Peer review status:ACCEPTED

16 Dec 2019Submitted to Biotechnology and Bioengineering
17 Dec 2019Submission Checks Completed
17 Dec 2019Assigned to Editor
03 Jan 2020Reviewer(s) Assigned
10 Feb 2020Review(s) Completed, Editorial Evaluation Pending
10 Feb 2020Editorial Decision: Revise Major
01 Apr 20201st Revision Received
02 Apr 2020Submission Checks Completed
02 Apr 2020Assigned to Editor
06 Apr 2020Reviewer(s) Assigned
19 Apr 2020Review(s) Completed, Editorial Evaluation Pending
19 Apr 2020Editorial Decision: Accept

Abstract

Clinical use of pancreatic beta islets for regenerative medicine applications requires mass production of functional cells. Current technologies are insufficient for large-scale production in a cost-efficient manner. Here, we evaluate advantages of a porous cellulose scaffold and demonstrate scale-up to a wicking-matrix bioreactor as a platform for culture of human endocrine cells. Scaffold modifications were evaluated in a multi-well platform to find the optimum surface condition for pancreatic cell expansion followed by bioreactor culture to confirm suitability. Preceding scale-up, cell morphology, viability and proliferation of primary pancreatic cells were evaluated. Two optimal surface modifications were chosen and evaluated further for insulin secretion, cell morphology and viable cell density for human induced pluripotent stem cell-derived pancreatic cells at different stages of differentiation. Scale-up was accomplished with uncoated, amine-modified cellulose in a miniature bioreactor, and insulin secretion and cell metabolic profiles were determined for 13 days. We achieved 10-fold cell expansion in the bioreactor along with a significant increase in insulin secretion compared with cultures on tissue-culture plastic. Our findings define a new method for expansion of pancreatic cells on wicking-matrix cellulose platform to advance cell therapy biomanufacturing for diabetes.