The demand for 1,3-propanediol (1,3-PDO) has increased sharply due to its role as a monomer for the synthesis of polytrimethylene terephthalate (PTT). Although Clostridium butyricum is considered to be one of the most promising bio-producers for 1,3-PDO, its low productivity hinders its application on industrial scale because of the longer time needed for anaerobic cultivation. In this study, an excellent mutant of C. butyricum (DL07) was obtained with high-level titer and productivity of 1,3-PDO, i.e. 104.78 g/L and 3.38 g/(L•h) vs. 94.23 g/L and 3.04