AUTHOREA
Log in
Sign Up
Browse Preprints
LOG IN
SIGN UP
Essential Maintenance
: All Authorea-powered sites will be offline 9am-10am EDT Tuesday 28 May
and 11pm-1am EDT Tuesday 28-Wednesday 29 May. We apologise for any inconvenience.
Janine Martin
Laboratory Technologist III
Cincinnati Childrens' Hospital Medical Center
Member of:
Cincinnati Children's Hospital Medical Center
Public Documents
1
Overestimation of Baseline Factor IX Activity in a Patient with Mild Hemophilia B by...
Michael Losos
and 4 more
June 04, 2020
Testing and screening for factor deficiency using one-stage clotting assays is the most prevalent methodology, but it is susceptible to misestimating a patient’s true coagulable state due to either the clot detection methodology or the aPTT reagents involved. In our case report, a 16-year-old with mild hemophilia B had a normal aPTT and factor IX activity using a new platform in the clinical laboratory. We evaluated the impact of instrument and aPTT reagents that differed in clot detection methodology, the activator and the source of phospholipid in one stage assays (OSA) for FIX:C and aPTT using plasma from this patient.