Figure 6: The inhibitory effects of COL-3 on TNF-α release are reproduced by either reducing glucose metabolism or lowering NADPH oxidase activity . (A) Quantification of TNF-α levels in microglial cells exposed or not to LPS (10 ng/ml, 24-h) in the presence or not of COL-3 (20 μM), APO (300 µM), or 2-DG (500 µM µM). Data are means ± SEM (n = 6), *p < 0.05 vs control.#p < 0.05 vs LPS. One-way ANOVA followed by Bonferroni post-hoc test. (B) Quantification of TNFα levels in microglial cells exposed or not to αSa (70 µg/ml, 24-h) in the presence or not of COL-3 (20 μM), APO (300 µM), or 2DG (500 µM µM). Data are means ± SEM (n = 6), *p < 0.05 vs control.#p < 0.05 vs αSa. A One-way ANOVA followed by Bonferroni post-hoc test.