This study presents the development and validation of the Dissociative Identity Inventory (DII-28), a novel self-report instrument designed to assess dissociative experiences and measure inclinations toward pathological traits. Drawing from existing literature, the inventory comprises 28 items carefully crafted to capture a broad spectrum of dissociative phenomena and pathological tendencies. A sample of 90 university students (53 male, 37 female) participated in the validation process. Rigorous validation procedures, including exploratory factor analysis and reliability testing, were conducted to evaluate the psychometric properties of the DII-28. Results revealed a robust three-factor structure, comprising Dissociative Experiences, Pathological Traits, and Identity Disturbances, collectively explaining a significant proportion of the variance. Internal consistency reliability analysis demonstrated high reliability across all factors, indicating the stability and coherence of the inventory. The findings suggest that the DII-28 is a promising tool for researchers and clinicians to assess dissociative phenomena and pathological traits comprehensively. However, limitations, such as the relatively small sample size and the use of convenience sampling, underscore the need for further research to validate the instrument in larger and more diverse populations. Overall, the development and validation of the DII-28 represent a significant step toward advancing our understanding and assessment of dissociative experiences and pathological traits.