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Ferric ion crosslinking-based 3D printing of graphene oxide and its evaluation as bio-scaffold in tissue engineering
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  • Renhao Lu,
  • Wuhua Zhang,
  • Yuting He,
  • Siyuan Zhang,
  • Qian Fu,
  • Yuan Pang,
  • Wei Sun
Renhao Lu
Tsinghua University
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Wuhua Zhang
Tsinghua University
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Yuting He
Chongqing University
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Siyuan Zhang
Tsinghua University
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Qian Fu
Chongqing University
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Yuan Pang
Tsinghua University
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Wei Sun
Tsinghua University
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Abstract

As a precursor of graphene, graphene oxide (GO) exhibits excellent mechanical, thermal, and electrical properties, besides appreciable biocompatibility in tissue engineering applications. However, the current GO-3D fabrication technology is still in need of optimization and simplification in order to ensure fine architecture and reasonable mechanical property, which would further promote the performance of GO as bio-scaffolds in cell or microorganism attachment and in material transformation. To address this issue, we proposed a GO ink, with appreciable rheological properties and excellent printing performance via high-speed centrifugation and ferric ion-assisted cross-linking. A woodpile structure with controllable micro-pores was produced by micro-extrusion-based 3D printing technology followed by an optimized freeze-drying process. Cellular adhesion and viability were verified by inoculation and culture of HepaRG cells using the fabricated GO 3D structure, thus suggesting ferric ion-assisted cross-linking and controllable pore distribution to improve the performance of GO construct as a bio-scaffold for in-vitro liver tissue models.

Peer review status:ACCEPTED

27 Apr 2020Submitted to Biotechnology and Bioengineering
05 May 2020Submission Checks Completed
05 May 2020Assigned to Editor
20 May 2020Reviewer(s) Assigned
01 Aug 2020Review(s) Completed, Editorial Evaluation Pending
01 Aug 2020Editorial Decision: Revise Major
28 Aug 20201st Revision Received
28 Aug 2020Submission Checks Completed
28 Aug 2020Assigned to Editor
01 Sep 2020Reviewer(s) Assigned
26 Sep 2020Review(s) Completed, Editorial Evaluation Pending
26 Sep 2020Editorial Decision: Accept