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Distribution and Immunomodulatory mechanisms of Programmed death(PD)-1 molecule , its ligand PD-L1and related cytokines in Cervical Cancer
  • Yuhong Lin,
  • Xiaoxian Wang,
  • Wei Lin
Yuhong Lin
Fuzhou First Hospital
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Xiaoxian Wang
Fuzhou First Hospital
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Wei Lin
Fuzhou First Hospital
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Abstract

PD1(programmed cell death protein 1), a molecule negatively regulated by immunity,is highly expressed in a wide variety of human malignancies making it an attractive immunotherapeutic target. PD-L1 is one of the ligands for PD-1 and expressed both on T cells and tumor cells. The purpose of this study was to investigate the expression levels of PD1, PD-L1, regulatory T cells (Tregs), identified as CD4+CD25+CD127-/low, and related cytokines during the development and progression of cervical cancer(CC). Through different groups to simulate the process of the occurrence and development of CC, peripheral blood of each group was collected, and different indicators were detected by ELISA and flow analysis. It was found that except for INF-γ, PD1, PD-L1, IL-2, IL6, IL-10, TGF-β expression increased gradually with the development of CC, and the differences between groups were statistically significant (P<0.05). This situation also appears on the tracking of preoperative CC patients and postoperative follow-up on test results. And Tregs in peripheral blood, compared with normal control(NC) group, the expression increased CC group, the PD1 expression in the Tregs membrane also increases at the same time. In CC tissues, we found an interesting phenomenon through multiplex immunohistochemical staining that CD8+ T cells mainly infiltrated in the tumor cell aggregation area, while CD4+ T cells mainly appeared around. Therefore, we speculated that CD8+ T cells mainly played an anti-tumor immune role in CC tumor tissues.