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Genotypic characterization of Mycobacterium bovis isolates from dairy cattle
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  • Elizabeth Melo,
  • Harrison Gomes,
  • Ícaro Santos,
  • José Augusto Afonso,
  • Marília Junqueira Franco,
  • Helio Langoni,
  • Antonio Paes,
  • Philip Suffys ,
  • Carla Mendonça
Elizabeth Melo
Federal Rural University of Pernambuco
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Harrison Gomes
Instituto Oswaldo Cruz, FIOCRUZ
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Ícaro Santos
Instituto Oswaldo Cruz, FIOCRUZ
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José Augusto Afonso
Universidade Federal Rural de Pernambuco
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Marília Junqueira Franco
UNESP-Univ. Estadual Paulista
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Helio Langoni
UNESP Campus de Botucatu
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Antonio Paes
UNESP-Univ. Estadual Paulista, Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Sciences, Botucatu, State of São Paulo, Brazil.
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Philip Suffys
Institute Oswaldo Cruz
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Carla Mendonça
Universidade Federal Rural de Pernambuco
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Abstract

Molecular diagnosis of bovine tuberculosis plays an essential role in the epidemiological knowledge of the disease. Bovine tuberculosis caused by Mycobacterium bovis represents a risk to human health. This study aimed to perform the genotypic characterization of M. bovis isolated from bovines diagnosed as tuberculosis from dairy herds in the state of Pernambuco, Brazil. Granulomas from 30 bovines were sent for microbiological culture and colonies compatible with Mycobacterium spp were obtained in at least one culture from 17/30 granulomas. All isolates were confirmed to be M. tuberculosis bovis by spoligotyping and 24loci MIRU-VNTR typing. While spoligotyping characterized the isolates as SB0121, SB0295, SB0852, SB0120 and an unclassified genotype, 24loci MIRU-VNTR rendered two clusters of two isolates each and 13 unique profiles. Loci ETR-A showed higher discriminatory power, and loci (ETR-B, ETR-C, MIRU16, MIRU27 and QUB26) showed moderate allelic diversity. This is the first study on genetic variability of the infectious agent cause of bovine TB in Pernambuco and demonstrates variability of strains in the state. Thus, it corroborates the importance of this microorganism as agent of bovine tuberculosis and its zoonotic potential, being this epidemiological tool determinant in the rigor of the sanitary practices of disease control in dairy herds.